Feeding olive flounder (Paralichthys olivaceus) with Lactococcus lactis BFE920 expressing the fusion antigen of Vibrio OmpK and FlaB provides protection against multiple Vibrio pathogens: A universal vaccine effect. Issue 114 (July 2021)

• Record Type: Journal Article
• Authors: Lee, Soon Ho; Beck, Bo Ram; Hwang, Seok-Hong; Song, Seong Kyu
• Is Part Of: Fish & shellfish immunology. Issue 114(2021)
• Page Start: 253
• Page End: 262
• Publication Date: 2021-07
• Subjects: Vibrio species -- Fusion antigen -- Feed vaccine -- Oral vaccine -- Lactococcus lactis BFE920 -- Olive flounder; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.05.007
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Vibriosis, an illness caused by the Vibrio bacteria species, results in significant economic loss in olive flounder farms. Here we present a novel anti- Vibrio feed vaccine protecting multiple strains of Vibrio pathogens, a universal vaccine effect. The vaccine was generated by engineering Lactococcus lactis BFE920 to express the fusion antigens of Vibrio outer membrane protein K (OmpK) and flagellin B subunit (FlaB). These antigen genes are highly conserved among Vibrio species. Olive flounder (7.1 ± 0.8 g and 140 ± 10 g) were fed the vaccine adsorbed to a regular feed (1 × 10 7 CFU/g) for one week with a 1-week interval, repeating three times (a triple boost). The vaccinated fish increased the significant levels of antigen-specific antibodies, T cell numbers ( CD4-1, CD4-2, and CD8α ), cytokine production ( T-bet and IFN-γ ), and innate immune responses ( TLR5M, IL-1β, and IL-12p40 ). Also, the survival rates of adult and juvenile fish fed the vaccine were significantly elevated when challenged with V. anguillarum, V. alginolyticus, and V. harveyi . In addition, weight gain rate and feed conversion ratio were improved in vaccinated fish. The feed vaccine protected multiple Vibrio pathogens, a universal vaccine effect, by activating innate and adaptive immune responses. This oral vaccine may be developed as an anti- Vibrio vaccine to protect against a broad spectrum of Vibrio pathogens. Highlights: Lactococcus lactis BFE920 was engineered to express the fusion protein that are highly conserved among Vibrio pathogens. LAB vaccine fed olive flounder were pretected from multiple Vibrio pathogens: a universal vaccine effect. The vaccine-fed fish increased both innate immunity and adaptive immunity. The vaccinated olive flounder improved the weight gain rate and feed conversion ratio.