Genome-wide screening of vaccine targets prioritization and reverse vaccinology aided design of peptides vaccine to enforce humoral immune response against Campylobacter jejuni. (June 2021)

• Record Type: Journal Article
• Authors: Zeb, Adnan; Ali, Syed Shujait; Azad, Abul Kalam; Safdar, Muhammad; Anwar, Zeeshan; Suleman, Muhammad; Nizam-Uddin, N.; Khan, Abbas; Wei, Dong-Qing
• Is Part Of: Computers in biology and medicine. Volume 133(2021)
• Publication Date: 2021-06
• Subjects: Campylobacter -- Subtractive proteomics -- Vaccine targets -- Multi-epitopes vaccine -- Immune simulation -- In silico cloning; Medicine -- Data processing -- Periodicals; Biology -- Data processing -- Periodicals; 610.285
• DOI: 10.1016/j.compbiomed.2021.104412
• Languages: English
• ISSNs: 0010-4825

Abstract

Abstract: Campylobacter jejuni, gram-negative bacteria, is an infectious agent of foodborne disease-causing bloody diarrhea, abdominal pain, fever, Guillain-Barré syndrome (GBS) and Miller Fisher syndrome in humans. Campylobacter spp. with multidrug resistance to fluoroquinolones, tetracycline, and erythromycin are reported. Hence, an effective vaccine candidate would provide long-term immunity against C. jejuni infections. Thus, we used a subtractive proteomics pipeline to prioritize essential proteins, which impart a critical role in virulence, replication and survival. Five proteins, i.e. Single-stranded DNA-binding protein, UPF0324 membrane protein Cj0999c, DNA translocase FtsK, 50S ribosomal protein L22, and 50S ribosomal protein L1 were identified as virulent proteins and selected for vaccine designing. We reported that the multi-epitopes subunit vaccine based on CTL, HTL and B-cell epitopes combination possess strong antigenic properties and associates no allergenic reaction. Further investigation revealed that the vaccine interacts with the immune receptor (TLR-4) and triggered the release of primary and secondary immune factors. Moreover, the CAI and GC contents obtained through codon optimization were reported to be 0.93 and 53% that confirmed a high expression in the selected vector. The vaccine designed in this study needs further scientific consensus and will aid in managing C. jejuni infections. Graphical abstract: Image 1 Highlights: Campylobacter jejuni, gram-negative bacteria, is an infectious agent of foodborne diseases in humans. For MDR species an effective vaccine candidate against long term immunity against C. jejuni infections. We used a subtractive proteomics pipeline to prioritize essential proteins, which impart a critical role in virulence. We reported that the multi-epitopes subunit vaccine which triggered the release of primary and secondary immune factors. The vaccine designed in this study needs further scientific consensus and will aid in managing C. jejuni infections.