Replication competence of canine distemper virus in cell lines expressing signaling lymphocyte activation molecule (SLAM) of goat, sheep and dog origin. (July 2021)

• Record Type: Journal Article
• Authors: Yadav, Ajay Kumar; Rajak, Kaushal Kishor; Kumar, Ashok; Bhatt, Mukesh; Chakravarti, Soumendu; Muthu, Sankar; Dubal, Z.B.; Khulape, Sagar; Yousuf, Raja Wasim; Rai, Vishal; Kumar, Bablu; Muthuchelvan, Dhanavelu; Gupta, Praveen Kumar; Singh, Rabindra Prasad; Singh, Rajkumar
• Is Part Of: Microbial pathogenesis. Volume 156(2021)
• Publication Date: 2021-07
• Subjects: SLAM -- Stable cell lines -- CDV -- Growth kinetics; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2021.104940
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Cellular receptors play an important role in entry and cell to cell spread of morbillivirus infections. The cells expressing SLAM and Nectin-4 have been used for successful and efficient isolation of canine distemper virus (CDV) in high titre. There are several methods for generation of cells expressing receptor molecules. Here, we have used a comparatively cheaper and easily available method, pcDNA 3.1 (+) for engineering Vero cells to express SLAM gene of goat, sheep and dog origin (Vero/Goat/SLAM (VGS), Vero/Sheep/SLAM (VSS) and Vero/Dog/SLAM (VDS), respectively). The generated cell lines were then compared to test their efficacy to support CDV replication. CDV could be grown in high titre in the cells expressing SLAM and a difference of log two could be recorded in virus titre between VDS and native Vero cells. Also, CDV could be grown in a higher titre in VDS as compared to VGS and VSS. The finding of this study supports the preferential use of SLAM expressing cells over the native Vero cells by CDV. Further, the higher titre of CDV in cells expressing dog-SLAM as compared to the cells expressing SLAM of non-CDV hosts (i.e. goat and sheep) points towards the preferential use of dog SLAM by the CDV and may be a plausible reason for differential susceptibility of small ruminants and Canines to CDV. Highlights: Vero cells were engineered to express SLAM protein of goat, sheep and dog origin (Vero/Goat/SLAM; VGS, Vero/Sheep/SLAM; VSS and Vero/Dog/SLAM; VDS) using pcDNA3.1 (+) expression vector. The methodology used for generation of stable cell lines is easy and cost effective. SLAM expression could be detected in all the engineered cells at genomic, transcript and protein level. CDV could be grown in higher titre in cells expressing SLAM as compared to native Vero cells. Higher virus titre could be recorded in cells expressing SLAM of dog as compared to the cells expressing SLAM of other hosts.