Differential regulation of oxidative stress, microbiota-derived, and energy metabolites in the mouse brain during sleep. Issue 12 (December 2021)

• Record Type: Journal Article
• Authors: Vallianatou, Theodosia; Lin, Weifeng; Bèchet, Nicholas B; Correia, Mario SP; Shanbhag, Nagesh C; Lundgaard, Iben; Globisch, Daniel
• Is Part Of: Journal of cerebral blood flow & metabolism. Volume 41:Issue 12(2021)
• Page Start: 3324
• Page End: 3338
• Publication Date: 2021-12
• Subjects: Brain metabolism -- energy metabolism -- gut-brain axis -- metabolomics -- oxidative stress; Cerebral circulation -- Periodicals; Brain -- Metabolism -- Periodicals; Brain -- Blood-vessels -- Periodicals; Cerebrovascular disease -- Periodicals; 612.824
• DOI: 10.1177/0271678X211033358
• Languages: English
• ISSNs: 0271-678X

Abstract

Sleep has evolved as a universal core function to allow for restorative biological processes. Detailed knowledge of metabolic changes necessary for the sleep state in the brain is missing. Herein, we have performed an in-depth metabolic analysis of four mouse brain regions and uncovered region-specific circadian variations. Metabolites linked to oxidative stress were altered during sleep including acylcarnitines, hydroxylated fatty acids, phenolic compounds, and thiol-containing metabolites. These findings provide molecular evidence of a significant metabolic shift of the brain energy metabolism. Specific alterations were observed for brain metabolites that have previously not been associated with a circadian function including the microbiome-derived metabolite ergothioneine that suggests a regulatory function. The pseudopeptide β -citryl-glutamate has been linked to brain development and we have now discovered a previously unknown regioisomer. These metabolites altered by the circadian rhythm represent the foundation for hypothesis-driven studies of the underlying metabolic processes and their function.