Multichromatic Control of Signaling Pathways in Mammalian Cells. Issue 5 (12th October 2020)

• Record Type: Journal Article
• Authors: Kramer, Markus M.; Mühlhäuser, Wignand W. D.; Weber, Wilfried; Radziwill, Gerald
• Is Part Of: Advanced biology. Volume 5:Issue 5(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-10-12
• Subjects: multichromatics -- optogenetics -- signaling pathways; Molecular biology -- Periodicals; Systems biology -- Periodicals; Biological systems -- Periodicals; Biotechnology -- Periodicals; Bioengineering -- Periodicals; Biomedical engineering -- Periodicals; 660.6
• DOI: 10.1002/adbi.202000196
• Languages: English
• ISSNs: 2701-0198

Abstract

Abstract: The precise control of signaling proteins is a prerequisite to decipher the complexity of the signaling network and to reveal and to study pathways involved in regulating cellular metabolism and gene expression. Optogenetic approaches play an emerging role as they enable the spatiotemporal control of signaling processes. Herein, a multichromatic system is developed by combining the blue light cryptochrome 2 system and the red/far‐red light phytochrome B system. The use of three wavelengths allows the orthogonal control of the RAF/ERK and the AKT signaling pathway. Continuous exposure of cells to blue light leads to activation of AKT while simultaneous pulses of red and far‐red light enable the modulation of ERK signaling in cells with constantly active AKT signaling. The optimized, orthogonal multichromatic system presented here is a valuable tool to better understand the fine grained and intricate processes involved in cell fate decisions. Abstract : A multichromatic system is developed for simultaneous and orthogonal control of two signaling pathways. The system enables fine‐tuning of amplitude, duration, and frequency of AKT‐signaling via optoAKT by blue light as well as RAF/ERK signaling via optoSOS by red/far‐red light.