Nuclear hyperpolarization of (1-13C)-pyruvate in aqueous solution by proton-relayed side-arm hydrogenation. Issue 5 (21st January 2021)

• Record Type: Journal Article
• Authors: Dagys, Laurynas; Jagtap, Anil P.; Korchak, Sergey; Mamone, Salvatore; Saul, Philip; Levitt, Malcolm H.; Glöggler, Stefan
• Is Part Of: Analyst. Volume 146:Issue 5(2021)
• Page Start: 1772
• Page End: 1778
• Publication Date: 2021-01-21
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an02389b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We employ parahydrogen induced polarization with side-Arm hydrogenation (PHIP-SAH) to polarize (1- 13 C)-pyruvate via a new pulsed magnetic resonance experiment. Abstract : We employ Parahydrogen Induced Polarization with Side-Arm Hydrogenation (PHIP-SAH) to polarize (1- 13 C)-pyruvate. We introduce a new method called proton-relayed side-arm hydrogenation (PR-SAH) in which an intermediate proton is used to transfer polarization from the side-arm to the 13 C-labelled site of the pyruvate before hydrolysis. This significantly reduces the cost and effort needed to prepare the precursor for radio-frequency transfer experiments while still maintaining acceptable polarization transfer efficiency. Experimentally we have attained on average 4.33% 13 C polarization in an aqueous solution of (1- 13 C)-pyruvate after about 10 seconds of cleavage and extraction. PR-SAH is a promising pulsed NMR method for hyperpolarizing 13 C-labelled metabolites in solution, conducted entirely in high magnetic field.