Suppression of interleukin 1α and interleukin 1β in human limbal epithelial cells cultured on the amniotic membrane stromal matrix. Issue 4 (1st April 2001)

• Record Type: Journal Article
• Authors: Solomon, Abraham; Rosenblatt, Mark; Monroy, Dagoberto; Ji, Zhonghua; Pflugfelder, Stephen C; Tseng, Scheffer C G
• Is Part Of: British journal of ophthalmology. Volume 85:Issue 4(2001)
• Page Start: 444
• Page End: 449
• Publication Date: 2001-04-01
• Subjects: amniotic membrane -- interleukin 1 -- inflammation -- corneal limbal epithelium; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.85.4.444
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : AIMS: Amniotic membrane (AM) transplantation reduces inflammation in a variety of ocular surface disorders. The aim of this study was to determine if AM stroma suppresses the expression of the IL-1 gene family in cultured human corneal limbal epithelial cells. METHODS: Human corneal limbal epithelial cells were cultured from limbocorneal explants of donor eyes on plastic or on the AM stroma. Transcript expression of IL-1α, IL-1β, IL-1 receptor antagonist (RA), and GAPDH was compared with or without addition of lipopolysaccharide to their serum-free media for 24 hours using RNAse protection assay (RPA). Their protein production in the supernatant was analysed by ELISA. RESULTS: Expression of IL-1α and IL-1β transcripts and proteins was significantly reduced by cells cultured on the AM stromal matrix compared with plastic cultures whether lipopolysaccharide was added or not. Moreover, expression of IL-1 RA by cells cultured in the lipopolysaccharide-free medium was upregulated by AM stromal matrix. The ratio between IL-1 RA and IL-1α protein levels in AM cultures was higher than in plastic cultures. CONCLUSIONS: AM stromal matrix markedly suppresses lipopolysaccharide induced upregulation of both IL-1α and IL-1β. These data may explain in part the effect of AM transplantation in reducing ocular surface inflammation, underscoring the unique feature of the AM as a substrate for tissue engineering.