AB0157 The secretory activity of abdominal adipose tissue in patients with rheumatoid arthritis. (23rd January 2014)

• Record Type: Journal Article
• Authors: Kontny, E.; Zielinska, A.; Maslinski, W.; Gluszko, P.
• Is Part Of: Annals of the rheumatic diseases. Volume 71(2012)Supplement 3
• Page Start: 646
• Page End: 646
• Publication Date: 2014-01-23
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2012-eular.157
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Objectives: To investigate spontaneous adipocytokine secretion by subcutaneous abdominal adipose tissue (SAAT) explants obtained from patients with rheumatoid arthritis (RA). Methods: SAAT explants were obtained by 18 G needle biopsy form 18 RA patients (16F/2M, the mean age 58, 5±3, 6 years) dispersed and cultured in vitro (100mg tissue/ml). Select adipocytokines: leptin, adiponectin, IL-6, IL-8 and macrophage migration inhibiting factor (MIF) concentrations were measured in culturesupernatants using ELISA and in sera of patients. Body mass index (BMI), disease activity scores (DAS 28), x-ray Larsen scores, routine blood tests and other demographic and clinical information were obtained. The association of BMI with duration of the disease, DAS 28, CRP levels, ESR, x-ray scores and concentrations of adipocytokines was analysed using linear regression. The spontaneous adipocytokine secretion from SAAT was compared with serum levels of measured variables. Results: Disease activity of RA patients was high (DAS28 = 5, 28±0, 27SD, CRP levels 21.0±5, 2 mg/l), mean disease duration 11, 8±2, 5 years, and patients BMI 26, 98±1.28. Patients were treated with methotrexate (13) or leflunomide (1) or sulphasalazine (2) with low doses of prednisone (7). None had received biologics. The SAAT concentrations of IL-6 (1042±242 pg/100mg), IL-8 (3044±833 pg/100mg), MIF (2597±614 pg/100mg) were significantly higher (p<0, 006) comparing to the serum levels of IL-6 (747±416 pg/ml), IL-8 (49, 4±36, 9 pg/ml), MIF (476±76 pg/ml). The SAAT leptin (1, 828±0, 29 ng/100mg) was significantly lower then the serum leptin levels (30, 185±5, 7 ng/ml, p<0.05). BMI values of patients correlated positively with the SAAT IL-8 concentrations (r=0, 57 p<0.05) and with the serum leptin (r=0, 58 p<0.05) level, and negatively with x-ray scores ((r= -0, 62 p<0.05). We found positive correlation of serum CRP levels with SAAT IL-6 (r=0, 66 p<0.05) but not with serum IL-6. X-ray scores correlated positively with the disease duration (r=0, 77 p<0.05) and negatively with the SAAT IL-8 (r=-0.53 p<0.05). Conclusions: Our data suggest that subcutaneous abdominal adipose tissue may modulate the systemic inflammatory processes in RA releasing pro and anti-inflammatory mediators. Further studies are needed to explore potential association between obesity, abdominal fat bioactivity and RA severity and progression Disclosure of Interest: None Declared