An odorant‐binding protein of Asian citrus psyllid, Diaphorina citri, participates in the response of host plant volatiles. Issue 7 (3rd April 2021)

• Record Type: Journal Article
• Authors: Liu, Xiao‐Qiang; Jiang, Hong‐Bo; Fan, Jia‐Yao; Liu, Tian‐Yuan; Meng, Li‐Wei; Liu, Yi; Yu, Hai‐Zhong; Dou, Wei; Wang, Jin‐Jun
• Is Part Of: Pest management science. Volume 77:Issue 7(2021)
• Page Start: 3068
• Page End: 3079
• Publication Date: 2021-04-03
• Subjects: Diaphorina citri -- odorant‐binding protein -- microscale thermophoresis -- RNAi -- EAG and behavioral assay; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.6352
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: Odorant‐binding proteins (OBPs) in insects contribute to the sensitivity of the olfactory system and connect external odorants to olfactory receptor neurons. Determination of the chemosensory functions in Diaphorina citri, a vector of the citrus Huanglongbing pathogen, may help in developing a potential target for pest management. RESULTS: Diaphorina citri showed dose‐dependent electroantennogram recording (EAG) responses to 12 host plant volatiles. A two‐choice behavioral trap experiment showed that four compounds (methyl salicylate, linalool, citral and R ‐(+)‐limonene) that elicited high EAG responses also had significant attraction to adults. The expression profiles induced by these compounds were detected in nine OBP genes, DcitOBP1–9 . DcitOBP3, DcitOBP6 and DcitOBP7 commonly showed significant upregulation or downregulation compared with the control. Microscale thermophoresis (MST) showed that the recombinant protein DcitOBP7 had high in vitro binding affinities ( K d < 10 μm ) to methyl salicylate, linalool and R ‐(+)‐limonene, and moderate binding affinity to citral with a K d value of 15.95 μm . Furthermore, RNA interference (RNAi)‐suppressed messenger RNA (mRNA) expression of DcitOBP7 resulted in a significant reduction in EAG activity and in adult D. citri behavioral responses to tested volatiles and the preferred host, Murraya paniculata . The hydrophilic residue Arg107 of DcitOBP7 may have a key role in binding odorants via formation of hydrogen bonds. CONCLUSION: These results show that DcitOBP7 plays an important role in the olfactory response. This finding may provide new insight into the functions of OBP families in D. citri and aid in the development of safe strategies for managing D. citri populations. © 2021 Society of Chemical Industry. Abstract : DcitOBP7 had high binding affinities for test volatiles in an in vitro binding assay. Knockdown of DcitOBP7 reduced electroantennogram recording sensitivities and behavioral responses of Diaphorina citri to test volatiles. © 2021 Society of Chemical Industry.