O07.4 Incidence of sexual behaviors and relationship to the urethral microbiota among men who have sex with men (MSM) in seattle. (14th July 2019)
- Record Type:
- Journal Article
- Title:
- O07.4 Incidence of sexual behaviors and relationship to the urethral microbiota among men who have sex with men (MSM) in seattle. (14th July 2019)
- Main Title:
- O07.4 Incidence of sexual behaviors and relationship to the urethral microbiota among men who have sex with men (MSM) in seattle
- Authors:
- Chambers, Laura
Hughes, James
Fredricks, David
Srinivasan, Sujatha
Morgan, Jennifer
Hoffman, Noah
Lowens, M
Tapia, Kenneth
Glick, Sara
Khosropour, Christine
Golden, Matthew
Manhart, Lisa - Abstract:
- Abstract : Background: Studies suggest that sexual behavior influences the composition of the male urethral microbiota, but this hypothesis has not been tested. Methods: From 12/2014–5/2018, we enrolled MSM with NGU attending an STD clinic into a cohort study. Men attended five in-clinic visits at 3-week intervals, collected weekly urine specimens at home, and reported daily antibiotics and sex on weekly diaries. We applied broad-range 16S rRNA gene PCR with deep sequencing to urine. We estimated incidence of insertive oral sex (IOS) only, condomless insertive anal intercourse (CIAI) only, and IOS with CIAI (IOS+CIAI) after NGU diagnosis using Poisson regression with robust standard errors. We estimated the association between urethral sexual exposures (referent group=none) in seven 3-day time windows before specimen collection and Shannon Index (diversity) and log10 number of bacterial species (richness) using generalized estimating equations, adjusting for recent antibiotics, age, race/ethnicity, HIV status, and PrEP use. For each exposure category, we tested whether all seven window coefficients were equal to zero (i.e., no overall association) using a Wald test. Results: Among 92 MSM with NGU, median age was 31 (interquartile range [IQR]=28–40); 55% were non-Hispanic white. They contributed 1, 095 person-weeks of behavioral data (median=12 diaries/man, IQR=12–13). Incidence of any sex, IOS only, CIAI only, and IOS+CIAI were 1.07 (95% confidence interval [CI]=0.93–1.24),Abstract : Background: Studies suggest that sexual behavior influences the composition of the male urethral microbiota, but this hypothesis has not been tested. Methods: From 12/2014–5/2018, we enrolled MSM with NGU attending an STD clinic into a cohort study. Men attended five in-clinic visits at 3-week intervals, collected weekly urine specimens at home, and reported daily antibiotics and sex on weekly diaries. We applied broad-range 16S rRNA gene PCR with deep sequencing to urine. We estimated incidence of insertive oral sex (IOS) only, condomless insertive anal intercourse (CIAI) only, and IOS with CIAI (IOS+CIAI) after NGU diagnosis using Poisson regression with robust standard errors. We estimated the association between urethral sexual exposures (referent group=none) in seven 3-day time windows before specimen collection and Shannon Index (diversity) and log10 number of bacterial species (richness) using generalized estimating equations, adjusting for recent antibiotics, age, race/ethnicity, HIV status, and PrEP use. For each exposure category, we tested whether all seven window coefficients were equal to zero (i.e., no overall association) using a Wald test. Results: Among 92 MSM with NGU, median age was 31 (interquartile range [IQR]=28–40); 55% were non-Hispanic white. They contributed 1, 095 person-weeks of behavioral data (median=12 diaries/man, IQR=12–13). Incidence of any sex, IOS only, CIAI only, and IOS+CIAI were 1.07 (95% confidence interval [CI]=0.93–1.24), 0.40 (95%CI=0.32–0.49), 0.10 (95%CI=0.07–0.15), and 0.40 (95%CI=0.30–0.52) episodes per person-week, respectively. Among 894 urine specimens (median=10 specimens/man, IQR=8–12), median diversity was 1.33 (IQR=0.76–1.99), and median richness was 14 species (IQR=9–23). Overall, CIAI only ( P <0.01 in each model) but not IOS only or IOS+CIAI in the prior 21 days was associated with diversity and log10-richness. Diversity and log10-richness were lower 1–3 days after and higher 16–18 days after CIAI only. Conclusion: Among MSM after NGU, CIAI only in the prior 21 days was independently associated with diversity and richness of the urethral microbiota. Disclosure: No significant relationships. … (more)
- Is Part Of:
- Sexually transmitted infections. Volume 95(2019)Supplement 1
- Journal:
- Sexually transmitted infections
- Issue:
- Volume 95(2019)Supplement 1
- Issue Display:
- Volume 95, Issue 1 (2019)
- Year:
- 2019
- Volume:
- 95
- Issue:
- 1
- Issue Sort Value:
- 2019-0095-0001-0000
- Page Start:
- A53
- Page End:
- A53
- Publication Date:
- 2019-07-14
- Subjects:
- gay bisexual and other men who have sex with men -- microbiota
Sexually transmitted diseases -- Periodicals
HIV infections -- Periodicals
616.951005 - Journal URLs:
- http://sti.bmj.com/ ↗
http://www.ncbi.nlm.nih.gov/pmc/journals/176/ ↗
http://www.bmj.com/archive ↗ - DOI:
- 10.1136/sextrans-2019-sti.143 ↗
- Languages:
- English
- ISSNs:
- 1368-4973
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 18190.xml