THE DETERMINATION OF SERUM IRON AFTER THE INTRAVENOUS INJECTION OF IRON-DEXTRAN. Issue 3 (May 1960)

• Record Type: Journal Article
• Authors: Webster, D.
• Is Part Of: Journal of clinical pathology. Volume 13:Issue 3(1960)
• Page Start: 246
• Page End: 248
• Publication Date: 1960-05
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.13.3.246
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : A rapid technique for the determination of iron in serum, after the intravenous injection of iron-dextran, is presented. Dithionite is used for the reduction of ferric to ferrous iron. The use of a detergent solution enables protein precipitation to be omitted. The iron reagent, 4: 7-diphenyl-1: 10-phenanthroline, and its ferrous complex are both soluble in the dilute detergent solution. Lipaemic serum can be used without affecting the results. The recovery of added ferric iron, as iron-dextran, is quantitative.