Deregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer. (25th February 2014)

• Record Type: Journal Article
• Authors: Rossi, Ana Flávia Teixeira; Duarte, Márcia Cristina; Poltronieri, Ayla Blanco; Valsechi, Marina Curado; Jorge, Yvana Cristina; de-Santi Neto, Dalísio; Rahal, Paula; Oliani, Sonia Maria; Silva, Ana Elizabete
• Other Names: D'Acquisto Fulvio Academic Editor.
• Is Part Of: Mediators of inflammation. Volume 2014(2014)
• Publication Date: 2014-02-25
• Subjects: Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1155/2014/478138
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Objective. Annexin-A1 ( ANXA1 /AnxA1) and galectin-1 ( LGALS1 /Gal-1) are mediators that play an important role in the inflammatory response and are also associated with carcinogenesis. We investigated mRNA and protein expression in precancerous gastric lesions that participate in the progression cascade to gastric cancer, such as intestinal metaplasia (IM) and gastric ulcer (GU). Methods. Quantitative real-time PCR (qPCR) and immunohistochemical techniques were used to analyze the relative quantification levels (RQ) of ANXA1 and LGALS1 mRNA and protein expression, respectively. Results. Increased relative expression levels of ANXA1 were found in 100% of cases, both in IM (mean RQ = 6.22 ± 0.06 ) and in GU (mean RQ = 6.69 ± 0.10 ). However, the LGALS1 presented basal expression in both groups (IM: mean RQ = 0.35 ± 0.07 ; GU: mean RQ = 0.69 ± 0.09 ). Immunohistochemistry revealed significant positive staining for both the AnxA1 and Gal-1 proteins in the epithelial nucleus and cytoplasm as well as in the stroma of the IM and GU groups (P < 0.05 ) but absence or low immunorectivity in normal mucosa. Conclusion . Our results bring an important contribution by evidencing that both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions, suggesting their involvement in the early stages of gastric carcinogenesis, possibly due to an inflammatory process in the gastric mucosa.