Arsenic trioxide exposure to ovarian carcinoma cells leads to decreased level of topoisomerase II and cytotoxicity. Issue 4 (1st July 2006)

• Record Type: Journal Article
• Authors: Askar, N.; Cirpan, T.; Toprak, E.; Karabulut, B.; Selvi, N.; Terek, M. C.; Uslu, R.; Sanli, U. A.; Goker, E.
• Is Part Of: International journal of gynecological cancer. Volume 16:Issue 4(2006)
• Page Start: 1552
• Page End: 1556
• Publication Date: 2006-07-01
• Subjects: arsenic trioxide (As2O3) -- cytotoxicity -- ovarian carcinoma cells -- topoisomerase II; Generative organs, Female -- Cancer -- Periodicals; 616.99465
• DOI: 10.1136/ijgc-00009577-200607000-00009
• Languages: English
• ISSNs: 1048-891X

Abstract

Abstract : The objective of this study was to investigate the effect of arsenic trioxide (As2 O3 ) on topoisomerase II levels using western blotting method on MDAH 2774 ovarian carcinoma cell culture. Experimental designs were established to determine the cytotoxic effects of As2 O3 on MDAH 2774 cells and the IC50 (fatal dose for the 50% of cells) value. Cytotoxicity experiments were carried out using various concentrations of As2 O3 . The 2, 3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and trypan blue dye–exclusion tests were used to evaluate cytotoxicity. Topoisomerase II expressions were investigated using western blotting method with various concentrations of As2 O3 . Densitometric analysis of topoisomerase 2 bands was carried out using Quantity One 1-D analysis software (Bio-Rad USA, Life Science Research, Hercules, CA). IC50 value of As2 O3 was found to be 5 × 10 −6 M for MDAH 2774 cells. When the bands were evaluated, it was observed that there was a decrease in topoisomerase II levels in MDAH 2774 cells with increasing concentrations of As2 O3 . It was also observed by the densitometric analysis that topoisomerase II expression ratios of MDAH 2774 cells were decreased by approximately 50% at this concentration. Topoisomerase II levels were significantly decreased with the increasing concentrations of As2 O3 . Inhibition of topoisomerase II enzyme was one of the antiproliferative influence mechanisms of As2 O3 .