Evaluation of repeatability for the automatic estimation of endothelial cell density in donor corneas. Issue 9 (12th April 2007)

• Record Type: Journal Article
• Authors: Ruggeri, Alfredo; Grisan, Enrico; Schroeter, Jan
• Is Part Of: British journal of ophthalmology. Volume 91:Issue 9(2007)
• Page Start: 1213
• Page End: 1215
• Publication Date: 2007-04-12
• Subjects: Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.2006.112763
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Background/Aims: The repeatability of an automatic system for estimation of endothelial cell density (ECD) from microscopy images in donor corneas was assessed. Methods: A computer program for the automatic ECD estimation from frequency analysis was previously presented. An evaluation of its repeatability was performed on a data set containing 200 corneas by assessing the accuracy and precision of automatic versus manual values. For each cornea, 2–21 images (1536 total) at 100× for automatic ECD and one image at 200× for manual ECD were available. Results: Accuracy of automatic ECDs was −45 (SD 99) cells/mm 2 (−2% (4%)). Precision of repeated automatic ECDs on the same cornea was 62 (30) cells/mm 2 (3% (1%)). The algorithm was also capable of identifying all non-processable images. Conclusion: The proposed automatic technique proved to be reliable for its routine use in a typical eye bank setting like the one considered here.