Stimulation of IL-8 release from epithelial cells by gas cooker PM10: a pilot study. Issue 3 (1st March 2001)

• Record Type: Journal Article
• Authors: Dick, C A J; Dennekamp, M; Howarth, S; Cherrie, J W; Seaton, A; Donaldson, K; Stone, V
• Is Part Of: Occupational and environmental medicine. Volume 58:Issue 3(2001)
• Page Start: 208
• Page End: 210
• Publication Date: 2001-03-01
• Subjects: indoor air pollution -- PM10 -- interleukin-8; Medicine, Industrial -- Periodicals; Environmental health -- Periodicals; 616.980305
• DOI: 10.1136/oem.58.3.208
• Languages: English
• ISSNs: 1351-0711

Abstract

Abstract : OBJECTIVE: To measure the effect of matter collected by a method that has a 50% efficiency for particles with an aerodynamic diameter of 10 μm (PM10 ), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO2 ). METHOD: Multiple indoor PM10 samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system. RESULTS: Treatment of A549 cells with PM10 generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO2 did not alter the concentration of IL-8. CONCLUSION: PM10 generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.