Assessment of direct versus indirect magnetic bead-based T-cell isolation procedures followed by magnetic bead-based DNA isolation. (19th July 2016)

• Record Type: Journal Article
• Authors: Rosenbaum, Anna; Bleck, Ellen; Schneider, Matthias; Pongratz, Georg; Vordenbäumen, Stefan
• Is Part Of: Lupus science & medicine. Volume 3(2016)Supplement 1
• Publication Date: 2016-07-19
• Subjects: T Cells -- magnetic beads -- DNA isolation -- epigenetics; Systemic lupus erythematosus -- Periodicals; 616.772005
• DOI: 10.1136/lupus-2016-000167
• Languages: English
• ISSNs: 2398-8851

Abstract

Abstract : Objective: To compare direct and indirect bead-based T-cell isolation followed by magnetic bead-based DNA isolation. Methods: T-cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead-based automated DNA isolation in 10 healthy subjects. Purity of T-cells, purity of DNA (by A260/A280 ratio measurement) and DNA concentration were assessed. Results: Direct and indirect labelling resulted in comparable T-cell purity (93.11±1.47% vs. 94.99±1.54%, p= 0.125) and DNA concentration per cell (50.97±14.15 ng/(mlxcell) vs. 49.53±13.62 ng/(mlxcell), p=0.492), while DNA purity was significantly higher after direct labelling (1.82±0.05 vs. 1.78±0.03, p=0.0488). Conclusions: Both direct and indirect magnetic bead-based T-cell selection may be used prior to magnetic bead-based DNA isolation procedures.