Normal and aberrant splicing of LMNA. Issue 4 (23rd January 2014)

• Record Type: Journal Article
• Authors: Luo, Yue-Bei; Mastaglia, Frank L; Wilton, Steve D
• Is Part Of: Journal of medical genetics. Volume 51:Issue 4(2014)
• Page Start: 215
• Page End: 223
• Publication Date: 2014-01-23
• Subjects: Molecular Genetics -- Muscle Disease -- Developmental -- Neuromuscular Disease; Medical genetics -- Periodicals; 616.042
• DOI: 10.1136/jmedgenet-2013-102119
• Languages: English
• ISSNs: 1468-6244

Abstract

Abstract : The LMNA gene gives rise to at least three isoforms (lamin A, C, lamin AΔ10) as a result of normal alternative splicing, regulated by cis- and trans- acting regulatory factors, as well as the 5′ and 3′ untranslated regions of the gene. The two main isoforms, lamin A and C, are constitutive components of the fibrous nuclear lamina and have diverse physiological roles, ranging from mechanical nuclear membrane maintenance to gene regulation. The clinical spectrum of diseases (called 'laminopathies') caused by LMNA mutations is broad, including at least eight well-characterised phenotypes, some of which are confined to the skeletal muscles or skin, while others are multisystemic. This review discusses the different alternatively spliced isoforms of LMNA and the regulation of LMNA splicing, as well as the subgroup of mutations that affect splicing of LMNA pre-mRNA, and also seeks to bridge the mis-splicing of LMNA at transcript level and the resulting clinical phenotypes. Finally, we discuss the manipulation of LMNA splicing by splice-switching antisense oligonucleotides and its therapeutic potential for the treatment of some laminopathies.