Routine and rapid enzyme linked immunosorbent assays for circulating anti-glomerular basement membrane antibodies. Issue 2 (February 1988)

• Record Type: Journal Article
• Authors: Wheeler, J; Simpson, J; Morley, A R
• Is Part Of: Journal of clinical pathology. Volume 41:Issue 2(1988)
• Page Start: 163
• Page End: 170
• Publication Date: 1988-02
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.41.2.163
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Microtitre plate modifications of the original tube enzyme linked immunosorbent assay (ELISA) of Wheeler and Sussman were used for the routine and rapid assays of anti-glomerular basement membrane antibodies in human sera. In a prospective study of 238 sera from 200 patients, the routine assay (about 24 hours) detected circulating antibodies in seven sera from three patients with active anti-glomerular basement membrane disease. The remaining sera, from patients with a variety of other glomerulonephropathies, were negative by the assay. The rapid assays took less than four hours, and in a retrospective study, detected anti-glomerular basement membrane antibodies in a range of 15 positive sera, with a level of discrimination similar to that observed in the routine assay and with no false positive/negative results.