Combining MALDI‐2 and transmission geometry laser optics to achieve high sensitivity for ultra‐high spatial resolution surface analysis. (12th March 2019)

• Record Type: Journal Article
• Authors: Spivey, Eric C.; McMillen, Josiah C.; Ryan, Daniel J.; Spraggins, Jeffrey M.; Caprioli, Richard M.
• Is Part Of: Journal of mass spectrometry. Volume 54:Number 4(2019)
• Page Start: 366
• Page End: 370
• Publication Date: 2019-03-12
• Subjects: high spatial resolution -- MALDI -- MALDI‐2 -- transmission geometry; Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.4335
• Languages: English
• ISSNs: 1076-5174

Abstract

Abstract: A transmission geometry optical configuration allows for smaller laser spot size to facilitate high‐resolution matrix‐assisted laser/desorption ionization (MALDI) mass spectrometry. This increase in spatial resolution (ie, smaller laser spot size) is often associated with a decrease in analyte signal. MALDI‐2 is a post‐ionization technique, which irradiates ions and neutrals generated in the initial MALDI plume with a second orthogonal laser pulse, and has been shown to improve sensitivity. Herein, we have modified a commercial Orbitrap mass spectrometer to incorporate a transmission geometry MALDI source with MALDI‐2 capabilities to improve sensitivity at higher spatial resolutions.