Sphingosine 1‐phosphate promotes the proliferation of olfactory ensheathing cells through YAP signaling and participates in the formation of olfactory nerve layer. Issue 9 (14th February 2020)

• Record Type: Journal Article
• Authors: Bao, Xiaomei; Xu, Xingxing; Wu, Qian; Zhang, Jingjing; Feng, Wenjin; Yang, Danlu; Li, Fayi; Lu, Sheng; Liu, Huitao; Shen, Xiya; Zhang, Fan; Xie, Changnan; Wu, Shiyang; Lv, Zhaoting; Wang, Wei; Li, Hongjuan; Fang, Yuanyuan; Wang, Ying; Teng, Honglin; Huang, Zhihui
• Is Part Of: Glia. Volume 68:Issue 9(2020)
• Page Start: 1757
• Page End: 1774
• Publication Date: 2020-02-14
• Subjects: olfactory ensheathing cells -- olfactory nerve layer -- proliferation -- S1P -- YAP; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.23803
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract: Olfactory ensheathing cells (OECs) are unique glial cells with axonal growth‐promoting properties in the olfactory epithelium and olfactory bulb, covering the entire length of the olfactory nerve. The proliferation of OECs is necessary for the formation of the presumptive olfactory nerve layer (ONL) during development and OECs transplantation. However, the molecular mechanism underlying the regulation of OEC proliferation in the ONL still remains unknown. In the present study, we examined the role of sphingosine 1‐phosphate (S1P) and S1P receptors (S1PRs) on OEC proliferation. Initially, reverse transcription‐PCR (RT‐PCR), western blot and immunostaining revealed that S1PRs were highly expressed in the OECs in vitro and in vivo. Furthermore, we found that S1P treatment promoted the proliferation of primary cultured OECs mediated by S1PR1. Mechanistically, yes‐associated protein (YAP) was required for S1P‐induced OEC proliferation through RhoA signaling. Finally, conditional knockout of YAP in OECs reduced OEC proliferation in ONL, which impaired the axonal projection and growth of olfactory sensory neurons, and olfactory functions. Taken together, these results reveal a previously unrecognized function of S1P/RhoA/YAP pathway in the proliferation of OECs, contributing to the formation of ONL and the projection, growth, and function of olfactory sensory neurons during development. Abstract : S1P promotes OEC proliferation through S1PR1. S1P promotes OEC proliferation via RhoA/YAP signaling. S1P/RhoA/YAP signaling is critical for the formation of olfactory nerve layer and function of olfactory system.