Picroside II Protects SH‐SY5Y Cells From Autophagy and Apoptosis Following Oxygen Glucose Deprivation/Reoxygen Injury by Inhibiting JNK Signal Pathway. Issue 12 (11th July 2019) - Wang, Tingting
ABSTRACT: To explore the neuroprotective effect of picroside (Picr) II on C‐Jun NH2‐terminal kinase (JNK) signal pathway after oxygen glucose deprivation/reoxygen (OGD/R) in SH‐SY5Y cells. In vitro, SH‐SY5Y cells were used to establish the OGD/R model, which was divided into the control group, model group, Picr group, and SP600125 (SP) group. Cellular viability was measured by CCK8. Cytotoxicity was assessed with LDH assay kit. Ad‐GFP‐mRFP‐LC3 was used to monitor autophagosome and autolysosome. Apoptoic cells were detected by Annexin V‐FITC/PI apoptosis detection kit. The expressions of phospho‐JNK and phospho‐c‐Jun were determined by western blot (WB) and immunofluorescence. The expressions of phospho‐MKK4, phospho‐Bcl‐2, Bax, Beclin‐1, and LC3 I/II were determined by WB. In the control group, only limited apoptosis and autophagy was observed, and the expression of associated proteins was very low. After OGD/R, the cellular viability of SH‐SY5Y cells was reduced, whereas the cytotoxicity, apoptosis, and autophagy were increased, accompanied with an increase of phospho‐MKK4, phospho‐JNK, phospho‐c‐Jun, phospho‐Bcl‐2, LC3 II, Beclin‐1, and Bax. During the reoxygen, treatment with Picr II or SP600125 could strengthen the cellular viability of SH‐SY5Y cells, but repress the cytotoxicity, apoptosis, autophagy, and the expressions of associated protein. OGD/R could induce apoptosis and autophagy of SH‐SY5Y cells by activating JNK signal pathway. Picr II could protect SH‐SY5Y cells from autophagy and apoptosis following OGD/R by inhibiting JNK signal pathway. Anat Rec, 302:2245–2254, 2019. © 2019 American Association for Anatomy