Greater ethanol inhibition of presynaptic dopamine release in C57BL/6J than DBA/2J mice: Role of nicotinic acetylcholine receptors. (22nd January 2015)

• Record Type: Journal Article
• Authors: Yorgason, J.T.; Rose, J.H.; McIntosh, J.M.; Ferris, M.J.; Jones, S.R.
• Is Part Of: Neuroscience. Volume 284(2015)
• Page Start: 854
• Page End: 864
• Publication Date: 2015-01-22
• Subjects: α-Ctx α-conotoxin -- aCSF artificial cerebral spinal fluid -- ANOVA analysis of variance -- AP-5 (2R)-amino-5-phosphonovaleric acid -- C57 C57BL/6J -- CPP conditioned place preference -- DBA DBA/2J -- DhβE dihydro-β-erythroidine hydrobromide -- I.P. intraperitoneal -- NAc nucleus accumbens -- nAChRs nicotinic acetylcholine receptors -- VTA ventral tegmental area; ethanol vulnerability -- phasic dopamine -- voltammetry -- C57BL/6 DBA/2 mice -- nucleus accumbens -- mecamylamine; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2014.10.052
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Ethanol modulates dopamine levels through a balance of excitation and inhibition. Ethanol inhibits dopamine release preferentially under high-frequency stimulations. DBA mice are less sensitive to ethanol inhibition than C57 mice. Nicotinic receptor antagonism blocks ethanol-mediated inhibition of dopamine. Abstract: The mesolimbic dopamine system, originating in the ventral tegmental area (VTA) and projecting to the nucleus accumbens (NAc), has been heavily implicated in the reinforcing effects of ethanol. Recent slice voltammetry studies have shown that ethanol inhibits dopamine release selectively during high-frequency activity that elicits phasic dopamine release shown to be important for learning and reinforcement. Presently, we examined ethanol inhibition of electrically evoked NAc dopamine in two mouse strains with divergent dopamine responses to ethanol, C57BL/6 (C57) and DBA/2J (DBA) mice. Previous electrophysiology and microdialysis studies have demonstrated greater ethanol-induced VTA dopaminergic firing and NAc dopamine elevations in DBA compared to C57 mice. Additionally, DBA mice have greater ethanol responses in dopamine-related behaviors, including hyperlocomotion and conditioned place preference. Currently, we demonstrate greater sensitivity of ethanol inhibition of NAc dopamine signaling in C57 compared to DBA mice. The reduced sensitivity to ethanol inhibition in DBA mice may contribute to the overall greater ethanol-induced dopamine signaling and related behaviors observed in this strain. NAc cholinergic activity is known to potently modulate terminal dopamine release. Additionally, ethanol is known to interact with multiple aspects of nicotinic acetylcholine receptor activity. Therefore, we examined ethanol-mediated inhibition of dopamine release at two ethanol concentrations (80 and 160 mM) during bath application of the non-selective nicotinic receptor antagonist mecamylamine, as well as compounds selective for the β2-(dihydro-β-erythroidine hydrobromide; DhβE) and α6-(α-conotoxin MII [H9A; L15A]) subunit-containing receptors. Mecamylamine and DhβE decreased dopamine release and reduced ethanol's inhibitory effects on dopamine in both DBA and C57 mice. Further, α-conotoxin also reduced the dopamine release and the dopamine-inhibiting effects of ethanol at the 80 mM, but not 160 mM, concentration. These data suggest that ethanol is acting in part through nicotinic acetylcholine receptors, or downstream effectors, to reduce dopamine release during high-frequency activity.