Enhanced consumption of salient solutions following pedunculopontine tegmental lesions. (22nd January 2015)

• Record Type: Journal Article
• Authors: MacLaren, D.A.A.; Markovic, T.; Daniels, D.; Clark, S.D.
• Is Part Of: Neuroscience. Volume 284(2015)
• Page Start: 381
• Page End: 399
• Publication Date: 2015-01-22
• Subjects: ChAT choline acetyltransferase -- DA dopamine -- DAB 3, 3′-diaminobenzidin -- DAT-KO knock out of the dopamine transporter -- Dtx–UII diphtheria toxin–urotensin II -- IAL interaural line -- NeuN neuronal nuclei -- PB phosphate buffer -- PBS phosphate-buffered saline -- PPTg pedunculopontine tegmental nucleus -- SN substantia nigra; pedunculopontine -- salience -- sucrose -- overconsumption -- Dtx–UII; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2014.09.075
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Rats with lesions of the pedunculopontine tegmentum consume significantly more 20% sucrose than controls. Excitotoxic, but not selective cholinergic lesions, produced overconsumption. Using a contact lickometer system it was found that overconsumption is not due to perseveration. Overconsumption only occurs for salient solutions—both sucrose and saline are only overconsumed when salient. An intact PPTg is important for the normal behavioral response to salient stimuli. Abstract: Rats with lesions of the pedunculopontine tegmental nucleus (PPTg) reliably overconsume high concentration sucrose solution. This effect is thought to be indicative of response-perseveration or loss of behavioral control in conditions of high excitement. While these theories have anatomical and behavioral support, they have never been explicitly tested. Here, we used a contact lickometer to examine the microstructure of drinking behavior to gain insight into the behavioral changes during overconsumption. Rats received either excitotoxic (ibotenic acid) damage to all PPTg neuronal subpopulations or selective depletion of the cholinergic neuronal sub-population (diphtheria toxin–urotensin II (Dtx–UII) lesions). We offered rats a variety of pleasant, neutral and aversive tastants to assess the generalizability and specificity of the overconsumption effect. Ibotenic-lesioned rats consumed significantly more 20% sucrose than sham controls, and did so through licking significantly more times. However, the behavioral microstructure during overconsumption was unaffected by the lesion and showed no indications of response-perseveration. Furthermore, the overconsumption effect did not generalize to highly consumed saccharin. In contrast, while only consuming small amounts of quinine solution, ibotenic-lesioned rats had significantly more licks and bursts for this tastant. Selective depletion of cholinergic PPTg neurons had no effect on consumption of any tastant. We then assessed whether it is the salience of the solution which determines overconsumption by ibotenic-lesioned rats. While maintained on free-food, ibotenic-lesioned rats had normal consumption of sucrose and hypertonic saline. After mild food deprivation ibotenic PPTg-lesioned rats overconsumed 20% sucrose. Subsequently, after dietary-induced sodium deficiency, lesioned rats consumed significantly more saline than controls. These results establish that it is the salience of the solution which is the determining factor leading to overconsumption following excitotoxic PPTg lesion. They also find no support for response-perseveration contributing to this effect. Results are discussed in terms of altered dopamine (DA) and salience signaling.