Environmentally relevant concentrations of glibenclamide induce oxidative stress in common carp (Cyprinus carpio). (April 2018)

• Record Type: Journal Article
• Authors: Guadalupe Martínez-Viveros, Ericka María; Islas-Flores, Hariz; Dublán-García, Octavio; Galar-Martínez, Marcela; SanJuan-Reyes, Nely; García-Medina, Sandra; Hernández-Navarro, María Dolores; Gómez-Oliván, Leobardo Manuel
• Is Part Of: Chemosphere. Volume 197(2018)
• Page Start: 105
• Page End: 116
• Publication Date: 2018-04
• Subjects: Oxidative damage -- Antioxidant enzymes -- Hyperglycemic agent -- Teleost; CAT Catalase -- CHP Cumene hydroperoxide -- CYP450 Cytochrome P450 -- EC50 Median effective concentration -- GLB Glibenclamide -- HPC Hydroperoxide content -- HPLC-MS/MS High performance liquid chromatography-tandem mass spectrometry -- LC50 Median lethal concentration -- LPX Lipid peroxidation -- MDA Malondialdehyde -- MEC Molar extinction coefficient -- OD Optical density -- PCC Protein carbonyl content -- SOD Superoxide dismutase -- TBARs Thiobarbituric acid reactive substances; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.01.020
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The hypoglycemic pharmaceutical glibenclamide (GLB) is widely used around the world. This medication is released into the environment by municipal, hospital and industrial wastewater discharges. Although there are reports of its environmental occurrence in the scientific literature, toxicity studies on aquatic species of commercial interest such as the common carp Cyprinus carpio are scarce. The present study aimed to evaluate the oxidative stress induced on C. carpio by environmentally relevant concentrations of GLB. Biomarkers of oxidative damage such as hydroperoxide content, lipid peroxidation and protein carbonyl content were evaluated as well as the activity of the antioxidant enzymes superoxide dismutase and catalase. The concentration of GLB was determined in water as well as in gill, liver, muscle, brain and blood of carp at 12, 24, 48, 72 and 96 h. The findings obtained in the study prove that GLB induces increases in biomarkers of oxidative damage and antioxidant enzyme activity in the teleost C. carpio, that this response is not concentration dependent and that the organs evaluated bioconcentrate this hypoglycemic agent. These findings permit us to conclude that the presence of GLB in water bodies represents a risk for aquatic species. Graphical abstract: Image Highlights: GLB is antidiabetic drug used around the world. GLB is released into water bodies. This work evaluated GLB-induced oxidative damage in Cyprinus carpio . GLB uptake is high in liver and muscle of C. carpio and lower in brain and gill. Exposure to GLB induced oxidative stress in C. carpio . Exposure to ng/L of GLB induces oxidative damage in fish.