Functional characterization of a protein inhibitor of activated STAT (PIAS) gene in Litopenaeus vannamei. Issue 94 (November 2019)

• Record Type: Journal Article
• Authors: Zhang, Shuang; Li, Chaozheng; Wang, Wei; Wang, Chenggui; Sun, Chengbo; Chan, Siuming; Shi, Lili
• Is Part Of: Fish & shellfish immunology. Issue 94(2019)
• Page Start: 417
• Page End: 426
• Publication Date: 2019-11
• Subjects: Protein inhibitor of activated STAT -- Signal transducer and activator of transcription -- Litopenaeus vannamei -- WSSV; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.09.007
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Protein inhibitor of activated STAT (PIAS) plays a critical role in the feedback modulation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway as a negative regulator in mammals and Drosophila, but the function of PIAS in crustaceans is still unclear. In this study, a PIAS termed LvPIAS was cloned and characterized from Litopenaeus vannamei . The full length of LvPIAS was 3065 bp, including a 2361 bp open reading frame (ORF) coding for a protein of 786 aa. LvPIAS expression was most abundant in muscle and could respond to the challenge of LPS, Vibrio parahaemolyticus, Staphhylococcus aureus, Poly I: C and white spot syndrome virus (WSSV). LvPIAS could be induced by the transcription factor LvSTAT, but LvPIAS could inhibit the transcriptional activity of LvSTAT to the LvPIAS promoter conversely, which indicated that there was a negative feedback loop between LvSTAT and LvPIAS. Furthermore, RNAi-mediated knockdown of LvPIAS shrimps showed higher survival rate to WSSV infection than those in the control group (dsGFP injection), suggesting that LvPIAS may play a negatively role against WSSV infection. Highlights: The protein inhibitor of activated STAT (PIAS) was identified from Litopenaeus vannamei. LvPIAS was a transcriptional target gene of LvSTAT. LvPIAS inhibited the transcriptional activity of LvSTAT to the LvPIAS promoter. Shrimp with knockdown of LvPIAS show enhanced resistance to WSSV.