AB0281 The Potential Cellular Marker for Disease Activity of Rheumatoid Arthritis: Circulating CD4+Cd161+ T Cells. (10th June 2014)

• Record Type: Journal Article
• Authors: Zhu, P.; Zhang, K.; Miao, J.
• Is Part Of: Annals of the rheumatic diseases. Volume 73:Supplement 2(2014)
• Page Start: 897
• Page End: 897
• Publication Date: 2014-06-10
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2014-eular.3453
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: Rheumatoid arthritis (RA) is a common autoimmune disease that is primarily driven by effector Tcells, in particular by Th17 cells which are contained within CD4+CD161+ Tcells. Objectives: We aim to evaluate the levels and cytokine secretion of circulating CD4+CD161+ T cells and Th17-related cytokines in patients with RA, and to elucidatetheir correlations with RA disease activity. Methods: Flow cytometry was used to analyze the surface phenotype and cytokine production of peripheral blood from 42 patients with RA and 11 healthy controls (HC). Plasma levels of Th17-related cytokines were measured by cytometric bead array (CBA). Additionally, disease activity was evaluated by the 28-joint Disease Activity Score (DAS28). Spearman's rank correlation and Receiver operating characteristic (ROC) curve were used for analyzing the relation of the indicators and disease activity. Results: Mainly circulating interleukin (IL)-17–producing cells were contained within the CD4+CD161+ T cells in patients with RA and HC. The levels of circulating CD4+CD161+ T cells, CD4+CD161+IL-17+IFN-γ+ cells (CD161+ Th17 cells), IL-17 and IL-6 in RA patient were significantly elevated and correlated strongly with disease activity indices. Accordingly, enhanced percentages of these indicators were observed in RA patients with active disease status (DAS28 >3.2) than in those with low disease status (DAS28 ≤3.2). In contrast, the percentages of circulating CD4+CD161+IL-17−IFN-γ+ cells (CD161+ Th1 cells) were significantly decreased and negatively correlated with disease activity indices in RA patients. ROC curve analysis identifiedthe composite indicator which was combined by the levels of CD4+CD161+ T cells and erythrocyte sedimentation rate (ESR) distinguished disease activity with 91.7% sensitivity and 94.4% specificity. Conclusions: Circulating CD4+CD161+ T cells may consider as a potentially cytologic biomarker of RA disease activity. Moreover, the composite indicator including CD4+CD161+ T cells and ESR may be a useful indicator for monitoring disease activity and treatment response in RA. Disclosure of Interest: None declared DOI: 10.1136/annrheumdis-2014-eular.3453