Hydrogen gas alleviates toxic effects of cadmium in Brassica campestris seedlings through up-regulation of the antioxidant capacities: Possible involvement of nitric oxide. (August 2019)

• Record Type: Journal Article
• Authors: Su, Nana; Wu, Qi; Chen, Hui; Huang, Yifan; Zhu, Zhengbo; Chen, Yahua; Cui, Jin
• Is Part Of: Environmental pollution. Volume 251(2019)
• Page Start: 45
• Page End: 55
• Publication Date: 2019-08
• Subjects: Cadmium stress -- Hydrogen gas -- Nitric oxide -- Hydrogen-rich water -- Brassica campestris; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.03.094
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Hydrogen gas (H2 ) has been shown as an important factor in plant tolerance to abiotic stresses, but the underlying mechanisms remain unclear. In the present study, the effects of H2 and its interaction with nitric oxide (NO) on alleviating cadmium (Cd) stress in Brassica campestris seedlings were investigated. NO donor (SNP) or hydrogen-rich water (HRW) treatment showed a significant improvement in growth of Cd-stressed seedlings. Cd treatment upregulated both endogenous NO and H2 (36% and 66%, respectively), and the increase of H2 was prior to NO increase. When treated with NO scavenger (PTIO) or NO biosynthesis enzyme inhibitors (L-NAME and Gln), HRW-induced alleviation under Cd stress was prevented. Under Cd stress, HRW pretreatment significantly enhanced the NO accumulation, and together up-regulated the activity of NR (nitrate reductase) and expression of NR . HRW induced lower reactive oxygen species (ROS), higher AsA content, enhanced activity of POD (peroxidase) and SOD (superoxide dismutase) in seedling roots were inhibited by PTIO, L-NAME and Gln. Through proteomic analysis, the level of 29 proteins were changed in response to H2 and NO-induced amelioration of Cd stress. Nearly half of them were involved in oxidation-reduction processes (about 20%) or antioxidant enzymes (approximately 20%). These results strongly indicate that in Cd-stressed seedlings, pretreatment with HRW induces the accumulation of H2 (biosynthesized or permeated), which further stimulates the biosynthesis of NO through the NR pathway. Finally, H2 and NO together enhance the antioxidant capabilities of seedlings in response to Cd toxicity. Graphical abstract: Image 1 Highlights: SNP and HRW treatments both improve Cd tolerance of cabbage seedlings. Cd increases the endogenous level of both H2 and NO. NO biosynthesis inhibitors and scavenger reverse HRW-induced improvement of Cd tolerance. HRW enhances the accumulation of NO and the activity of NR under Cd stress. Antioxidant enzymes are the response factors during the improvement of Cd tolerance induced by HRW and SNP. Abstract : Nitric oxide was involved in the alleviation of cadmium stress mediated by hydrogen gas in Brassica campestris seedlings.