Physical and biological performances of a semi-resorbable barrier membrane based on silk fibroin-glycerol-fish collagen material for guided bone regeneration. (November 2021)

• Record Type: Journal Article
• Authors: Pripatnanont, Prisana; Chankum, Chanokpim; Meesane, Jirut; Thonglam, Jutakan
• Is Part Of: Journal of biomaterials applications. Volume 36:Number 5(2022)
• Page Start: 930
• Page End: 942
• Publication Date: 2021-11
• Subjects: Biocompatibility -- collagen -- guided bone regeneration -- semi-resorbable barrier membrane silk fibroin; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/08853282211025781
• Languages: English
• ISSNs: 0885-3282

Abstract

The fragility of silk fibroin film is a drawback to being used as a barrier membrane. Semi-resorbable barrier membranes maintain function longer than a resorbable membrane and no need to be removed. The study aimed to fabricate semi-resorbable membranes using silk fibroin with glycerol plasticizer (Group A), immobilized with fish collagen (Group B), and then characterized, in vitro biocompatibility tested, and compared with a commercial collagen membrane (Group C). Group B showed more roughness (0.2155 µm) than Group A (0.1424 µm). Group A was more hydrophilic (76.75° ± 3.07°) and more stiffness (28.93% ± 15.56%) than Group B (112.67° ± 1.94°, 42.10% ± 11.46%) and C (54.79% ± 13.44%) without significant difference. Group C had a significantly higher ( p < 0.05) swelling degree and less degradation rate than others. Group A showed significantly highest ( p < 0.05) cell proliferation. Group C showed more alkaline phosphatase activity than others but no significant difference in osteocalcin and Alizarin Red activity on day 21. The semi-resorbable membrane based on silk fibroin-glycerol possessed good physical and mechanical properties, and well-supported osteoblastic cell proliferation and differentiation.