Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia. (February 2018)

• Record Type: Journal Article
• Authors: Sankaran, Revathy; Show, Pau Loke; Lee, Sze Ying; Yap, Yee Jiun; Ling, Tau Chuan
• Is Part Of: Bioresource technology. Volume 250(2018)
• Page Start: 306
• Page End: 316
• Publication Date: 2018-02
• Subjects: Liquid biphasic flotation -- Integration -- Lipase -- Fermentation -- Separation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.11.050
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Integration of fermentation and liquid biphasic flotation for lipase separation. Incorporate fermentation and extraction process of lipase from B. cepacia . Flotation system give higher cell growth and lipase production at shorter period. Integration process showed high yield of lipase with 95.73%. This system exhibited its potential for new prospects in industrial production. Abstract: Liquid Biphasic Flotation (LBF) is an advanced recovery method that has been effectively applied for biomolecules extraction. The objective of this investigation is to incorporate the fermentation and extraction process of lipase from Burkholderia cepacia using flotation system. Initial study was conducted to compare the performance of bacteria growth and lipase production using flotation and shaker system. From the results obtained, bacteria shows quicker growth and high lipase yield via flotation system. Integration process for lipase separation was investigated and the result showed high efficiency reaching 92.29% and yield of 95.73%. Upscaling of the flotation system exhibited consistent result with the lab-scale which are 89.53% efficiency and 93.82% yield. The combination of upstream and downstream processes in a single system enables the acceleration of product formation, improves the product yield and facilitates downstream processing. This integration system demonstrated its potential for biomolecules fermentation and separation that possibly open new opportunities for industrial production.