Non-targeted metabolomics reveals alterations in liver and plasma of gilt-head bream exposed to oxybenzone. (November 2018)

• Record Type: Journal Article
• Authors: Ziarrusta, Haizea; Mijangos, Leire; Picart-Armada, Sergio; Irazola, Mireia; Perera-Lluna, Alexandre; Usobiaga, Aresatz; Prieto, Ailette; Etxebarria, Nestor; Olivares, Maitane; Zuloaga, Olatz
• Is Part Of: Chemosphere. Volume 211(2018)
• Page Start: 624
• Page End: 631
• Publication Date: 2018-11
• Subjects: Oxybenzone -- Gilt-head bream -- Non-targeted metabolomics; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.08.013
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The extensive use of the organic UV filter oxybenzone has led to its ubiquitous occurrence in the aquatic environment, causing an ecotoxicological risk to biota. Although some studies reported adverse effects, such as reproductive toxicity, further research needs to be done in order to assess its molecular effects and mechanism of action. Therefore, in the present work, we investigated metabolic perturbations in juvenile gilt-head bream ( Sparus aurata ) exposed over 14 days via the water to oxybenzone (50 mg/L). The non-targeted analysis of brain, liver and plasma extracts was performed by means of UHPLC-qOrbitrap MS in positive and negative modes with both C18 and HILIC separation. Although there was no mortality or alterations in general physiological parameters during the experiment, and the metabolic profile of brain was not affected, the results of this study showed that oxybenzone could perturb both liver and plasma metabolome. The pathway enrichment suggested that different pathways in lipid metabolism (fatty acid elongation, α-linolenic acid metabolism, biosynthesis of unsaturated fatty acids and fatty acid metabolism) were significantly altered, as well as metabolites involved in phenylalanine and tyrosine metabolism. Overall, these changes are signs of possible oxidative stress and energy metabolism modification. Therefore, this research indicates that oxybenzone has adverse effects beyond the commonly studied hormonal activity, and demonstrates the sensitivity of metabolomics to assess molecular-level effects of emerging contaminants. Graphical abstract: Image 1 Highlights: The first metabolomic study exposing fish to the UV filter oxybenzone. Oxybenzone perturbed the liver and plasma metabolome, but not the brain metabolome. Phenylalanine, tyrosine and lipid metabolisms altered based on pathway enrichment. Signs of possible energy metabolism modification and oxidative stress were observed. Metabolomics is sensitive to assess molecular-level adverse effects of xenobiotics.