Linear and branched polyacrylates as a delivery platform for peptide-based vaccines. (September 2016)

• Record Type: Journal Article
• Authors: Chandrudu, Saranya; Bartlett, Stacey; Khalil, Zeinab G; Jia, Zhongfan; Hussein, Waleed M; Capon, Robert J; Batzloff, Michael R; Good, Michael F; Monteiro, Michael J; Skwarczynski, Mariusz; Toth, Istvan
• Is Part Of: Therapeutic delivery. Volume 7:Number 9(2016)
• Page Start: 601
• Page End: 609
• Publication Date: 2016-09
• Subjects: clinical isolates -- group A streptococcus -- nanoparticles -- opsonization -- peptide vaccine -- polyacrylates -- polymer–peptide conjugate -- single immunization -- vaccine delivery; Drug delivery systems -- Periodicals; Therapeutics -- Periodicals; 615.6
• DOI: 10.4155/tde-2016-0037
• Languages: English
• ISSNs: 2041-5990

Abstract

Aim: Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system.Results: Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer–peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates.Conclusion: We have successfully demonstrated that submicron-sized polymer–peptide conjugates were capable of inducing strong humoral immune responses after single immunization.