LC–MS determination of triazolam and its hydroxy metabolites in mouse dried blood spots: application to transgenic mouse pharmacokinetic studies. (July 2017)

• Record Type: Journal Article
• Authors: Kole, Prashant; Rao, Abhijith; Kurawattimath, Vishwanath; Mandlekar, Sandhya
• Is Part Of: Bioanalysis. Volume 9:Number 13(2017)
• Page Start: 987
• Page End: 1000
• Publication Date: 2017-07
• Subjects: dried blood spot -- drug–drug interaction -- LC–MS/MS -- metabolites -- method validation -- pharmacokinetic study -- surrogate biomatrix -- triazolam; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2017-0067
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: The objective of this study was to develop a LC–MS/MS method for the simultaneous determination of triazolam (TRZ) and its two hydroxy metabolites in transgenic mouse dried blood spots (DBS) using BALB/c mouse blood as a surrogate biomatrix.Methodology/Results: The DBS method involved spotting volume of 10 μl using Ahlstrom 226 sample collection cards. A 'whole spot' analysis (6-mm punch) involved extraction of analytes using water and acetonitrile containing an internal standard. DBS samples were analyzed by a validated LC–MS/MS method with a run time of 4 min.Conclusion: This validated LC–MS/MS method using DBS extraction was applied to quantitation of TRZ, α-hydroxytriazolam and 4-hydroxytriazolam in a CYP3A4 transgenic mouse oral pharmacokinetic study of TRZ.