Rapid identification of M. abscessus and M. massiliense by MALDI-TOF mass spectrometry with a comparison to sequencing methods and antimicrobial susceptibility patterns. (November 2013)

• Record Type: Journal Article
• Authors: Tseng, Sung-Pin; Teng, Shih-Hua; Lee, Pei-Shan; Wang, Chu-Feng; Yu, Jau-Song; Lu, Po-Liang
• Is Part Of: Future microbiology. Volume 8:Number 11(2013:Nov.)
• Page Start: 1381
• Page End: 1389
• Publication Date: 2013-11
• Subjects: antibiotic susceptibility -- matrix-assisted laser desorption ionization-time-of-flight -- multilocus sequence analysis -- multilocus sequence typing -- Mycobacterium abscessus complex; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.13.115
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: Development of the matrix-assisted laser desorption ionization-TOF (MALDI-TOF) mass spectrometry method to rapidly identify Mycobacteria abscessus and Mycobacteria massiliense from M. abscessus complex in clinical microbiology laboratories.Materials & methods: Of 128 M. abscessus complex clinical isolates, sequence analysis identified 64 as M. massiliense and 64 as M. abscessus . MALDI-TOF mass spectrometry with clustering analysis created a model to differentiate these two species. Multilocus sequence typing was used to confirm our model.Results: Using a model containing five signals, 100% species recognition was achieved for 50 strains of each species. The sequence type (ST) cluster of M. abscessus was distinct from the cluster of M. massiliense . ST1 was prevalent (59%) among M. abscessus isolates, and ST117 was common (41%) among M. massiliense isolates.Conclusion: Molecular methods are more costly and time-consuming and may not be available in the clinical microbial laboratory. Subsequently, MALDI-TOF analysis could be a rapid identification method in the future.