Applying molecular modelling and experimental studies to develop molecularly imprinted polymer for domoic acid enrichment from both seawater and shellfish. (May 2018)

• Record Type: Journal Article
• Authors: Ao, Junjie; Gu, Jiaping; Yuan, Tao; Li, Dan; Ma, Yuning; Shen, Zhemin
• Is Part Of: Chemosphere. Volume 199(2018)
• Page Start: 98
• Page End: 106
• Publication Date: 2018-05
• Subjects: Domoic acid -- Molecular modelling -- Molecularly imprinted polymer -- Solid-phase extraction -- Seawater -- Shellfish; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.02.005
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: A highly selective sample cleanup method using molecularly imprinted polymers (MIP) was developed for the enrichment of domoic acid (DA, an amnesic shellfish toxin) from both seawater and shellfish samples. Molecular modelling was firstly applied to screening a suitable functional monomer and optimize the polymer preparation. Theoretical results were in a good agreement with those of the experimental studies. MIP was prepared by precipitation polymerization using 1, 3, 5-pentanetricarboxylic acid and 2-(Trifluoromethyl)acrylic acid as the template molecule and functional monomer, respectively. The morphology and molecular structure of MIP were revealed by scanning electron microscope (SEM) and fourier transform infrared spectroscopy (FTIR), respectively. The obtained MIP showed high affinity and selectivity for DA with binding site numbers of 0.875 mg g −1 and an average association constant of 0.219 L mg −1 evaluated by adsorption experiments. The developed molecularly imprinted solid-phase extraction (MISPE) column achieved satisfied adsorption rate (99.2%) and recovery (71.2%) with relative standard deviation (RSD) less than 1.0%, which is more stable and precise than the C18, SAX, and HLB columns. Finally, the determination method for DA in both seawater and shellfish samples was then successfully established and validated using MISPE coupled with high-performance liquid chromatography-ultraviolet detection (HPLC-UV). The method limit of detection was 20 μg L −1 and 50 μg kg −1 for seawater and shellfish, respectively. This study demonstrates that molecular modelling is a useful tool to screening functional monomer and optimize polymer preparation. It provides an innovative polymer for trace DA monitoring in both seawater and shellfish. Graphical abstract: Image 1 Highlights: New molecularly imprinted polymer (MIP) was developed for domoic acid enrichment. Molecular modelling was firstly applied to screening for functional monomer. The obtained MIP showed high affinity and selectivity for domoic acid. MISPE column is more stable and precise than C18, SAX, and HLB columns. The developed MISPE-HPLC method successfully applied in both seawater and shellfish.