Green synthesis of bioactive polysaccharide-capped gold nanoparticles for lymph node CT imaging. (1st February 2018)

• Record Type: Journal Article
• Authors: Uthaman, Saji; Kim, Hyeon Sik; Revuri, Vishnu; Min, Jung-Joon; Lee, Yong-kyu; Huh, Kang Moo; Park, In-Kyu
• Is Part Of: Carbohydrate polymers. Volume 181(2018)
• Page Start: 27
• Page End: 33
• Publication Date: 2018-02-01
• Subjects: X-ray computed tomography -- Mannan -- Gold nanoparticles -- Contrast agent -- Green chemistry; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.10.042
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: M-GNPs as CT contrast agent for lymph node imaging. M-GNPs were spherical nanoparticles with an average diameter of 9.18 ± 0.71 nm. M-GNPs exhibited mannose receptor mediated endocytosis in antigen presenting cells. Local administration of M-GNPs led to significantly enhanced X-ray imaging of popliteal lymph nodes. Abstract: The development of biologically targeted contrast agents for X-ray computed tomography (CT) imaging remains a major challenge. Here, we investigated a green chemistry-based synthesis of lymph node-targeted mannan-capped gold nanoparticles (M-GNPs) as a CT contrast agent. In this study, mannan was used as a reducing and stabilizing agent for gold nanoparticles (AuNPs). M-GNPs were readily internalized by antigen-presenting cells (APCs) through mannose receptors-mediated endocytosis. The M-GNPs, which had a spherical morphology, had an average diameter of 9.18 ± 0.71 nm and surface plasmon resonance (SPR) absorption spectra with maximal absorption at 522 nm. The M-GNPs displayed a concentration-based X-ray attenuation property with a maximum Hounsfield unit (HU) value of 303.2 ± 10.83. The local administration of M-GNPs led to significantly enhanced X-ray contrast for the imaging of popliteal lymph nodes. These findings demonstrated that M-GNPs can be used as biologically targeted contrast agents for CT imaging.