Kit-dependent discrepancy in D16S539 and general considerations for database matches. (May 2018)

• Record Type: Journal Article
• Authors: Zajac, Barbara Karolina; Zehner, Richard; Scheiper, Stefanie; Weissenberger, Melanie
• Is Part Of: Forensic science international. Volume 34(2018)
• Page Start: 148
• Page End: 151
• Publication Date: 2018-05
• Subjects: D16S539 -- STR-kit dependent allele discrepancies -- Database matches -- Indels; Forensic genetics -- Periodicals; Génétique légale -- Périodiques; Forensic genetics; Electronic journals; Periodicals; 614.1
• DOI: 10.1016/j.fsigen.2018.02.012
• Languages: English
• ISSNs: 1872-4973

Abstract

Abstract: Throughout the last decade more companies have been offering multiplex PCR kits for forensic STR typing. As a consequence, it has been demonstrated, that an observed genotype may unexpectedly vary at a single locus when different STR kits have been used. Analysing STR profiles which have to be entered in a national database, unknown or undetected primer binding site mutations, insertions or deletions within the flanking region of STR loci may hinder matches and therefore have far-reaching consequences. The current study is a further example indicating that sequence variations in flanking regions are a common problem within STR typing which should not be underestimated. A deletion of 16 nucleotides close to the primer binding site downstream of the repeat sequence resulted in deviant genotypes at the D16S539 locus according to different STR kits used.