Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp. (1st April 2020)

• Record Type: Journal Article
• Authors: Zhao, Ping; Li, Xia; Wang, Ying; Yan, Lanyi; Guo, Lanping; Huang, Luqi; Gao, Wenyuan
• Is Part Of: Carbohydrate polymers. Volume 233(2020)
• Publication Date: 2020-04-01
• Subjects: PC Polygonatum cyrtonema -- PK Polygonatum kingianum -- PO Polygonatum odoratum -- PS Polygonatum sibiricum -- HPGPC high-performance gel permeation chromatography -- RID refractive index detector -- PMP Precolumn 1-phenyl-3-methyl-5-pyrazolone -- FTIR Fourier transform infrared -- Ara arabinose -- Fru fructose -- Gal galactose -- GalA galacturonic acid -- Glc glucose -- GlcA galacturonic acid -- Rha rhamnose -- Xyl xylose -- Man mannose -- Fuc fucose; Polygonatum -- Polysaccharides -- Saccharide mapping -- Fructans -- Pectins; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.115836
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Polysaccharides from PS, PC and PK were composed of pectins and fructans. Polysaccharides from PO mainly consisted of fructans. Polysaccharides from cultivated-type of Polygonatum spp. had less pectins. Esterification were found in pectins from Polygonatum spp. Abstract: Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra. Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 10 5 Da (P1) and the other was 2.8–5.4 × 10 3 Da (P2); while the polysaccharides from PO displayed only one main peak (P2). The analysis of monosaccharide composition and HPGPC saccharide mapping proved that P1 and P2 were composed of pectins and fructans, respectively. The FTIR spectra indicated that these polysaccharides had different degrees of esterification. This study provided a systematic profiling of polysaccharides of Polygonatum spp. and was helpful in understanding the varied functions of different Polygonatum spp., based on chemical composition.