Functional characterization of galectin-3 from Nile tilapia (Oreochromis niloticus) and its regulatory role on monocytes/macrophages. Issue 95 (December 2019)

• Record Type: Journal Article
• Authors: Niu, Jinzhong; Huang, Yu; Liu, Xinchao; Luo, Guoling; Tang, Jufen; Wang, Bei; Lu, Yishan; Cai, Jia; Jian, Jichang
• Is Part Of: Fish & shellfish immunology. Issue 95(2019)
• Page Start: 268
• Page End: 276
• Publication Date: 2019-12
• Subjects: Galectin-3 -- Nile tilapia -- Monocytes/macrophages -- Inflammation -- Phagocytosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.10.043
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Galectin-3 is a kind of β-galactoside-binding lectin involved in host defense against pathogen infection. However, the immune functions of fish galectin-3 remain poorly understood. In this study, the roles of a fish galectin-3 (OnGal-3) from Nile tilapia ( Oreochromis niloticus ) on the binding activity on bacterial pathogens or PAMPs, the agglutinating activity on bacterial pathogens and the regulatory effects on monocytes/macrophages activity were investigated. After in vitro challenge of Streptococcus agalactiae and Aeromonas hydrophila, OnGal-3 expressions were significantly up-regulated in monocytes/macrophages. In addition, recombinant OnGal-3(rOnGal-3) protein showed strong binding activity on bacterial pathogens or PAMPs. Also, rOnGal-3 agglutinated Gram-positive and Gram-negative bacteria. Moreover, rOnGal-3 could induce the inflammatory factors expressions in monocytes/macrophages and enhance phagocytosis and respiratory burst activity of monocytes/macrophages. These results suggest that fish galectin-3 participates in anti-bacterial immune response through recognizing pathogens and modulating monocytes/macrophages activity. Highlights: OnGal-3 expressions in monocytes/macrophages were up-regulated after S. agalactiae and A. hydrophila stimulation. rOnGal-3 could bind Gram-positive/Gram-negative bacteria and polysaccharides, as well as aggregate bacterial pathogen. rOnGal-3 could enhance the phagocytosis and respirator bursts activity of monocytes/macrophages.