Mangiferin suppresses allergic asthma symptoms by decreased Th9 and Th17 responses and increased Treg response. (October 2019)

• Record Type: Journal Article
• Authors: Yun, Chenxia; Chang, Ming; Hou, Guanghan; Lan, Taijin; Yuan, Hebao; Su, Zhiheng; Zhu, Dan; Liang, Weiping; Li, Qiaofeng; Zhu, Hongyan; Zhang, Jian; Lu, Yi; Deng, Jiagang; Guo, Hongwei
• Is Part Of: Molecular immunology. Volume 114(2019:Oct.)
• Page Start: 233
• Page End: 242
• Publication Date: 2019-10
• Subjects: AHR airway hyper-responsiveness -- BALF bronchoalveolar lavage fluid -- Cldyn dynamic compliance -- DEX dexamethasone -- ELISA enzyme-linked immunosorbent assay -- EOS eosinophils -- H&E hematoxylin and eosin -- IHC immunohistochemistry -- OVA ovalbumin -- PAS periodic acid-schiff -- PBS phosphate buffer saline -- Re resistance of expiration -- Ri resistance of the lung -- Th1 CD4+ helper T-cell type 1 -- Th2 CD4+ helper T-cell type 2 -- Th9 CD4+ helper T-cell type 9 -- Th17 CD4+ helper T-cell type 17 -- Treg regulatory T cells; Asthma -- Mangiferin -- Th9 cell -- Th17 cell -- Treg cell; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2019.07.025
• Languages: English
• ISSNs: 0161-5890

Abstract

Graphical abstract: Highlights: Mangiferin exhibits anti-asthma activity. Functional role of anti-asthma in attenuating EOS and goblet cells infiltration. Mangiferin regulates Th9, Th17 and Treg cells responses. Abstract: Mangiferin is the major bioactive ingredient in the leaves of Mangifera indica L., Aqueous extract of such leaves have been traditionally used as an indigenous remedy for respiratory diseases including cough and asthma in Traditional Chinese Medicine. Mangiferin was shown to exert its anti-asthmatic effect by modulating Th1/Th2 cytokines imbalance via STAT6 signaling pathway. However, compelling evidence indicated that subtypes of T helpers and regulatory T cells other than Th1/Th2 were also involved in the pathogenesis of asthma. In current study, we investigated the effects of mangiferin on the differentiation and function of Th9, Th17 and Treg cells in a chicken egg ovalbumin (OVA)-induced asthmatic mouse model. Mangiferin significantly attenuated the symptoms of asthma attacks, reduced the total number of leukocytes, EOS and goblet cells infiltration in lung. Simultaneously, treatment with mangiferin remarkably decreased the proportion of Th9 and Th17 cells; reduced the levels of IL-9, IL-17A; inhibited the expression of PU.1 and RORγt in lung. However, the proportion of Treg cells, the expression of IL-10, TGF-β1 and Foxp3 were increased by mangiferin. Our data suggest that mangiferin exerted anti-asthmatic effect through decreasing Th9 and Th17 responses and increasing Treg response in OVA-induced asthmatic mouse model.