Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila. Issue 94 (November 2019)

• Record Type: Journal Article
• Authors: Edirisinghe, S.L.; Dananjaya, S.H.S.; Nikapitiya, Chamilani; Liyanage, T.D.; Lee, Kyoung-Ah; Oh, Chulhong; Kang, Do-Hyung; De Zoysa, Mahanama
• Is Part Of: Fish & shellfish immunology. Issue 94(2019)
• Page Start: 558
• Page End: 565
• Publication Date: 2019-11
• Subjects: Aeromonas hydrophila -- Mucin -- Edwardsiella piscicida -- Immunostimulant -- Pectin -- Spirulina maxima; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.09.054
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In this study, we demonstrate the enhanced disease resistance and positive immunomodulation of novel pectin isolated from Spirulina maxima (SmP) in zebrafish model. Zebrafish larvae exposed to SmP had significantly ( p < 0.05) higher cumulative percent survival (CPS) at 25 (44.0%) and 50 μg/mL (67.0%) against Edwardsiella piscicida compared to the control. However, upon Aeromonas hydrophila challenge, SmP exposed larvae at 50 μg/mL had slightly higher CPS (33.3%) compared to control group (26.7%). SmP supplemented zebrafish exhibited the higher CPS against E. piscicida (93.3%) and A. hydrophila (60.0%) during the early stage of post-infection (<18 hpi). qRT-PCR results demonstrated that exposing (larvae) and feeding (adults) of SmP, drive the modulation of a wide array of immune response genes. In SmP exposed larvae, up-regulation of the antimicrobial enzyme ( lyz : 3.5-fold), mucin ( muc5.1 : 2.84, muc5.2 : 2.11 and muc5.3 : 2.40-fold), pro-inflammatory cytokines ( il1β : 1.79-fold) and anti-oxidants ( cat : 2.87 and sod1 : 1.82-fold) were identified. In SmP fed adult zebrafish (gut) showed >2-fold induced pro-inflammatory cytokine ( il1β ) and chemokines ( cxcl18b, ccl34a.4 and ccl34b.4 ). Overall results confirmed the positive modulation of innate immune responses in larval stage and it could be the main reason for developing disease resistance against E. piscicida and A. hydrophila . Thus, non-toxic, natural and biodegradable SmP could be considered as the potential immunomodulatory agent for sustainable aquaculture. Graphical abstract: Image 1 Highlights: Spirulina maxima based pectin (SmP) exposed zebrafish larvae enhances the diseases resistance. SmP induces the mucin 5.1 and mucin 5.3 in zebrafish larvae and gut, respectively. Biodegradable SmP could be a potential immunostimulant.