Combining autohydrolysis and ionic liquid microwave treatment to enhance enzymatic hydrolysis of Eucalyptus globulus wood. (March 2018)

• Record Type: Journal Article
• Authors: Rigual, Victoria; Santos, Tamara M.; Domínguez, Juan Carlos; Alonso, M. Virginia; Oliet, Mercedes; Rodriguez, Francisco
• Is Part Of: Bioresource technology. Volume 251(2018)
• Page Start: 197
• Page End: 203
• Publication Date: 2018-03
• Subjects: Pretreatment combination -- Eucalyptus wood -- Microwave ionic liquid pretreatment -- Fractal dimension -- Lacunarity; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.12.034
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Autohydrolysis followed by ionic liquid treatment enhanced enzymatic digestibility. Fractal dimension and enzymatic digestibility are directly proportional parameters. Lacunarity and enzymatic digestibility are inversely proportional parameters. Eucalyptus lignin surface deposition did not lower enzymatic digestibility. Abstract: The combination of autohydrolysis and ionic liquid microwave treatments of eucalyptus wood have been studied to facilitate sugar production in a subsequent enzymatic hydrolysis step. Three autohydrolysis conditions (150 °C, 175 °C and 200 °C) in combination with two ionic liquid temperatures (80 °C and 120 °C) were compared in terms of chemical composition, enzymatic digestibility and sugar production. Morphology was measured (using SEM) and the biomass surface was visualized with confocal fluorescence microscopy. The synergistic cooperation of both treatments was demonstrated, enhancing cellulose accessibility. At intermediate autohydrolysis conditions (175 °C) and low ionic liquid temperature (80 °C), a glucan digestibility of 84.4% was obtained. Using SEM micrographs, fractal dimension (as a measure of biomass complexity) and lacunarity (as a measure of homogeneity) were calculated before and after pretreatment. High fractals dimensions and low lacunarities correspond to morphologically complex and homogeneous samples, that are better digested by enzyme cocktails.