Abundance of antibiotic resistance genes and bacterial community composition in wild freshwater fish species. (April 2018)

• Record Type: Journal Article
• Authors: Marti, Elisabet; Huerta, Belinda; Rodríguez-Mozaz, Sara; Barceló, Damià; Marcé, Rafael; Balcázar, Jose Luis
• Is Part Of: Chemosphere. Volume 196(2018)
• Page Start: 115
• Page End: 119
• Publication Date: 2018-04
• Subjects: Bacterial community composition -- Antibiotic resistance genes -- Aquatic environments -- Wild fishes; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.12.108
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: This study was aimed to determine the abundance of four antibiotic resistance genes ( bla TEM, ermB, qnrS and sul I), as well as bacterial community composition associated with the intestinal mucus of wild freshwater fish species collected from the Foix and La Llosa del Cavall reservoirs, which represent ecosystems with high and low anthropogenic disturbance, respectively. Water and sediments from these reservoirs were also collected and analyzed to determine the pollution level by antibiotics. The bla TEM gene was only detected in brown trout and Ebro barbel, which were collected from La Llosa del Cavall reservoir. In contrast, the sul I and qnrS genes were only detected in common carp, which were collected from the Foix reservoir. Although the ermB gene was also detected in common carp, the values were below the limit of quantification. Likewise, water and sediment samples from the Foix reservoir had higher concentrations and more classes of antibiotics than those from La Llosa del Cavall. Pyrosequencing analysis of 16S rRNA genes revealed significant differences in bacterial communities associated with the intestinal mucus of fish species. Therefore, these findings suggest that anthropogenic activities are not only increasing the pollution of aquatic environments, but also contributing to the emergence and spread of antibiotic resistance in organisms that inhabit such environments. Highlights: Antibiotic pollution in two reservoirs was investigated. Antibiotic resistance in fish species from these reservoirs was analyzed. The sul I and qnrS genes were detected in fish from the reservoir with higher pollution load. Significant differences were observed in bacterial community composition between fish species.