Characterization of a novel polysaccharide from the leaves of Moringa oleifera and its immunostimulatory activity. (October 2018)

• Record Type: Journal Article
• Authors: Dong, Zhuping; Li, Chao; Huang, Qiang; Zhang, Bin; Fu, Xiong; Liu, Rui Hai
• Is Part Of: Journal of functional foods. Volume 49(2018)
• Page Start: 391
• Page End: 400
• Publication Date: 2018-10
• Subjects: Moringa oleifera leaves -- Polysaccharides -- Purification -- Structure -- Immunomodulatory; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.09.002
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: A novel polysaccharide MOP-2 was purified from Moringa oleifera leaves. The chemical structure of MOP-2 was characterized for the first time. MOP-2 could activate immunomodulatory response of RAW264.7 cells. MOP-2 could be developed as a potential immunoregulatory agent in functional foods. Abstract: A novel polysaccharide (MOP-2) was extracted and purified from the leaves of Moringa oleifera by hot water extraction and chromatographic purification. Structural characterization revealed that MOP-2 had an average molecular weight of 155.35 kDa and consisted of arabinose, glucose and galactose with molar percentages of 35.8, 6.67, and 57.53%, respectively. The backbone chain of MOP-2 was proven to be →3, 6)-β-D-Gal p (1→ and →6)-β-D-Gal p- (1→, and the side chains contained →5)-α-L-Ara f -(1→, α-L-Ara f -(1→, and α-D-Glc p -(1→. In vitro immunomodulatory assay revealed that MOP-2 could significantly enhance the proliferation and pinocytic capacity of the RAW264.7 macrophage cells. Furthermore, MOP-2 promoted the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by activating mRNA expressions of iNOS, IL-6 and TNF-α. These findings suggest that MOP-2 can be developed as a novel natural immunoregulatory agent in functional foods.