Inflammatory and oxidative injury is induced in cardiac and pulmonary tissue following fipronil exposure in Japanese quail: mRNA expression of the genes encoding interleukin 6, nuclear factor kappa B, and tumor necrosis factor-alpha. (August 2019)

• Record Type: Journal Article
• Authors: Khalil, Samah R.; Mohammed, Wafaa A.; Zaglool, Asmaa W.; Elhady, Walaa M.; Farag, Mayada R.; El sayed, Shafika A.M.
• Is Part Of: Environmental pollution. Volume 251(2019)
• Page Start: 564
• Page End: 572
• Publication Date: 2019-08
• Subjects: Cytokines -- Fipronil -- Interleukin 6 -- Japanese quail -- Nuclear factor kappa B -- Tumor necrosis factor-α; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.05.012
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The phenylpyrazole insecticide, fipronil, isused for the eradication of insects in agriculture, which also exposes various non-target groups such as birds and animals. Our aim was to assess the cardiac and pulmonary consequences of sub-acute administration of fipronil ( 1 ∕5 LD50 ; 2.26 mg/kg) in the Japanese quail for fifteen days and to determine the tissue recovery over a period of 60 days. Fipronil exposure led to a significant decrease in the body weight of the treated birds. Its exposure also induced cardiac and pulmonary damage of varying degrees. Fipronil increased the lipid peroxide (LPO) and nitric oxide (NO) contents as well as indices of tissue injury in the serum of exposed birds. Furthermore, it decreased the antioxidant indices in both the organs. Most of these changes gradually reversed and the histological changes, particularly of the heart, reversed completely by day-60 of recovery. Furthermore, alterations in the mRNA gene expressions of Nuclear factor kappa B (NF-κB), Interleukin 6 (IL-6), and Tumor necrosis factor-alpha (TNF-α) were monitored by quantitative polymerase chain reaction (RT-PCR). In both the tissues, a significant up-regulation of the transcripts was recorded after fipronil administration, which was reversed during the recovery period in the heart tissue except for TNF-α, while the transcripts in the lung tissue declined non-significantly. This study showed that the exposure of Japanese quail to fipronil has a profound negative impact on heart and lung including oxidative injury and tissue inflammation. Fipronil can induce the activity of NF-κB inflammatory -signaling pathway that play a role in the associated tissue inflammation. Although most of the cardiac changes could be reversed after a recovery period of sixty days, the pulmonary changes did not reverse much. Graphical abstract: Image 1 Highlights: Fipronil exposure has a negative influence on the heart and lung of Japanese quail. Fipronil mediated oxidative injury in both organs. Fipronil induced up-regulation of inflammation encoding genes mRNA in both organs. Heart tissue alterations could be reversed after a recovery period of 60 days. Lung tissue profound impairments could not be reversed after a recovery period of 60 days. Abstract : Fipronil has a negative impact on heart and lung tissue in male Japanese quail. Most of the cardiac changes could be reversed but not the pulmonary after a recovery period of 60 days.