Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents. (January 2018)

• Record Type: Journal Article
• Authors: Koutinas, Michalis; Yiangou, Chrystalleni; Osório, Natália M.; Ioannou, Katerina; Canet, Albert; Valero, Francisco; Ferreira-Dias, Suzana
• Is Part Of: Bioresource technology. Volume 247(2018)
• Page Start: 496
• Page End: 503
• Publication Date: 2018-01
• Subjects: EL ethyl lactate -- rROL heterologous sn-1, 3-regioselective Rhizopus oryzae lipase -- CRL Candida rugosa lipase -- GC Gas chromatography -- FFA free fatty acids -- CALB Candida antarctica lipase B; Ethyl lactate -- Esterification -- Lipase -- Novozym 435 -- Operational stability; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.09.130
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Different solvents were tested for lactate and ethanol esterification. Chloroform and hexane were the most effective solvents in low acid concentrations. Novozym 435 and Lipozyme RM IM could be efficiently applied in the reaction. Novozym 435 retained higher activity in chloroform as compared to hexane. Non-commercial enzymes exhibited significant activity towards hydrolysis. Abstract: This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01–0.1 M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent's Log P increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.