Anti-melanogenic and anti-oxidant activities of ethanol extract of Kummerowia striata: Kummerowia striata regulate anti-melanogenic activity through down-regulation of TRP-1, TRP-2 and MITF expression. (2019)

• Record Type: Journal Article
• Authors: Lee, Jae Yeon; Cho, Young-Rak; Park, Ju Hyoung; Ahn, Eun-Kyung; Jeong, Wonsik; Shin, Hyoung Seok; Kim, Mi-Sun; Yang, Seung Hwan; Oh, Joa Sub
• Is Part Of: Toxicology reports. Volume 6(2019)
• Page Start: 10
• Page End: 17
• Publication Date: 2019
• Subjects: L-DOPA L-3, 4-dihydroxyphenylalanine -- TRP-1 tyrosinase-related protein 1 -- MITF microphthalmia-associated transcription factor -- ABTS 2, 2ʹ-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt -- DPPH l 2, 2-diphenyl-1-picrylhydrazyl -- EKS ethanol extract of K. striata -- DMEM Dulbecco's Modified Eagle Medium -- FBS fetal bovine serum -- COSY correlation spectroscopy -- HSQC heteronuclear single quantum coherence -- HMBC heteronuclear multiple bond correlation -- ESI electrospray ionization -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide -- DMSO dimethyl sulfoxide -- α-MSH α-Melanocyte-stimulating hormone -- BHA butylated hydroxyanisole -- RT-PCR reverse transcription-polymerase chain reaction -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis; Kummerowia striata -- Anti-oxidant -- Melanin -- p-coumaric acid -- Quercetin; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2018.11.005
• Languages: English
• ISSNs: 2214-7500

Abstract

Highlights: Ethanol extract of Kummerowia striata is a potent antioxidant. It inhibits melanin synthesis by downregulating tyrosinase and related proteins. It may be used in cosmetics for skin whitening and reducing wrinkles. Abstract: Kummerowia striata ( K. striata ) is used as a traditional medicine for inflammation-related therapy. To determine whether it has beneficial anti-melanogenic and anti-oxidant activities, we investigated the biological activities of the ethanol extract of Kummerowia striata (EKS) using a variety of in vitro and cell culture model systems. The anti-melanogenic activity was assessed in B16F10 melanoma cells in terms of melanin synthesis and in vitro tyrosinase inhibitory activity. The anti-oxidant assays were performed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2ʹ-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). EKS showed strong anti-oxidant activities in DPPH and ABTS assays. The mRNA transcription levels and protein expression levels of tyrosinase, tyrosinase-related protein 1, tyrosinase-related protein 2, and microphthalmia-associated transcription factor decreased in a dose-dependent manner with EKS treatment. Additionally, EKS did not affect cell viability at different concentrations used in this study, indicating that the mechanism of action of EKS-mediated inhibition of melanin synthesis does not involve cytotoxicity. Also, we confirmed that p-coumaric acid and quercetin are important compounds for anti-melanogenesis and antioxidant properties of EKS. Collectively, our findings demonstrate for the first time that EKS possesses anti-melanogenic and anti-oxidant activities. Further evaluation and development of EKS as a functional supplement or cosmetic may be useful for skin whitening and reducing wrinkles.