SpBAG1 promotes the WSSV infection by inhibiting apoptosis in mud crab (Scylla paramamosain). Issue 94 (November 2019)

• Record Type: Journal Article
• Authors: Ma, Xiaomeng; Tang, Xixiang; Lin, Shanmeng; Gong, Yi; Tran, Ngoc Tuan; Zheng, Huaiping; Ma, Hongyu; Aweya, Jude Juventus; Zhang, Yueling; Li, Shengkang
• Is Part Of: Fish & shellfish immunology. Issue 94(2019)
• Page Start: 852
• Page End: 860
• Publication Date: 2019-11
• Subjects: Mud crab -- WSSV -- SpBAG1 -- Apoptosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.10.013
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Bcl-2 associated athanogene-1 (BAG1) is involved in various signalling pathways including apoptosis, cell proliferation, gene transcriptional regulation and signal transduction in animals. However the functions of BAG1 during the antiviral response of mud crab Scylla paramamosain is still unclear. In this study, the mud crab BAG1 ( Sp BAG1) was characterized to consist of 1761 nucleotides, containing an opening frame of 630bp encoding 209 amino acids with an ubiquitin domain and a BAG1 domain. Sp BAG1 was found to be significantly up-regulated at 6 h–24 h, but down-regulated from 48 h–72 h in the hemocytes of mud crab after challenge with white spot syndrome virus (WSSV). RNAi knock-down of Sp BAG1 significantly reduced the copies of WSSV and increased the apoptotic rate in mud crabs. The finding from this study suggested that Sp BAG1 could promote the WSSV infection by inhibiting apoptosis in mud crab. Therefore, to the best of our knowledge, this is the first study demonstrating the role of Sp BAG1 as a novel apoptosis inhibitor to promote virus infection in mud crab. Highlights: Sp BAG1 was up-regulated in mud crab in the early stage of WSSV infection. RNAi of Sp BAG1 can increase the apoptotic rate in mud crab. Sp- BAG1 acts as an apoptosis inhibitor to promote virus infection in mud crab