Sol-gel approach for extracting highly versatile aspirin and its metabolites using MISPE followed by GC–MS/MS analysis. (April 2016)

• Record Type: Journal Article
• Authors: Bhatia, Tejasvi; Gupta, Manoj Kumar; Singh, Pratibha; Chauhan, Abhishek; Saxena, Prem Narain; Mudiam, Mohana Krishna Reddy
• Is Part Of: Bioanalysis. Volume 8:Number 8(2016)
• Page Start: 795
• Page End: 805
• Publication Date: 2016-04
• Subjects: aspirin and its metabolites -- GC–MS/MS -- molecular imprinted polymer -- sol-gel polymerization; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.16.28
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: Aspirin is known to be a salicylate drug widely used as an analgesic, antipyretic and anti-inflammatory drug.Methodology: Sol-gel based nanosized molecularly imprinted polymer (nMIP) has been synthesized for extraction of aspirin and its metabolites in urine followed by GC–MS/MS analysis.Results: Binding affinity of nMIP and nonimprinted polymer was found to be in the range of 70–95% and 29–45%, respectively. LOD and LOQ of aspirin and its metabolites were found to be in the range of 0.63–2.4 ng/ml and 2.07–7.68 ng/ml, respectively.Conclusion: The developed method was found to be applicable for routine analysis of aspirin and its metabolites in biological samples.