Automated DBS microsampling, microscale automation and microflow LC–MS for therapeutic protein PK. (April 2016)

• Record Type: Journal Article
• Authors: Zhang, Qian; Tomazela, Daniela; Vasicek, Lisa A; Spellman, Daniel S; Beaumont, Maribel; Shyong, BaoJen; Kenny, Jacqueline; Fauty, Scott; Fillgrove, Kerry; Harrelson, Jane; Bateman, Kevin P
• Is Part Of: Bioanalysis. Volume 8:Number 7(2016)
• Page Start: 649
• Page End: 659
• Publication Date: 2016-04
• Subjects: automation -- DBS -- LC -- microflow -- microsampling -- MS -- PD -- PK -- sample preparation -- therapeutic protein; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2015-0006
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim : Reduce animal usage for discovery-stage PK studies for biologics programs using microsampling-based approaches and microscale LC–MS.Methods & results: We report the development of an automated DBS-based serial microsampling approach for studying the PK of therapeutic proteins in mice. Automated sample preparation and microflow LC–MS were used to enable assay miniaturization and improve overall assay throughput. Serial sampling of mice was possible over the full 21-day study period with the first six time points over 24 h being collected using automated DBS sample collection. Overall, this approach demonstrated comparable data to a previous study using single mice per time point liquid samples while reducing animal and compound requirements by 14-fold.Conclusion: Reduction in animals and drug material is enabled by the use of automated serial DBS microsampling for mice studies in discovery-stage studies of protein therapeutics.