Myeloid related protein 8 and 14 secretion reflects phagocyte activation and correlates with disease activity in juvenile idiopathic arthritis treated with autologous stem cell transplantation. Issue 3 (1st March 2003)

• Record Type: Journal Article
• Authors: Wulffraat, N M; Haas, P J; Frosch, M; de Kleer, I M; Vogl, T; Brinkman, D M C; Quartier, P; Roth, J; Kuis, W
• Is Part Of: Annals of the rheumatic diseases. Volume 62:Issue 3(2003)
• Page Start: 236
• Page End: 241
• Publication Date: 2003-03-01
• Subjects: myeloid related proteins -- juvenile idiopathic arthritis -- stem cells; ASCT, autologous stem cell transplantation -- CHAQ, Child Health Assessment Questionnaire -- CK, creatine kinase -- CRP, C reactive protein -- DMARDs, disease modifying antirheumatic drugs -- ELISA, enzyme linked immunosorbent assay -- ESR, erythrocyte sedimentation rate -- JIA, juvenile idiopathic arthritis -- MAS, macrophage activation syndrome -- MRP, myeloid related protein -- MTX, methotrexate -- SLE, systemic lupus erythematosus -- TNF, tumour necrosis factor; Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/ard.62.3.236
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Objectives: To determine whether myeloid related proteins (MRP8/MRP14), a complex of two S100 proteins related to neutrophil and monocyte activation, might be used as a marker for disease activity, and as an early indicator of relapse in juvenile idiopathic arthritis. Patients and methods: A group of 12 patients who underwent an autologous haematopoietic stem cell transplantation (ASCT) for refractory juvenile idiopathic arthritis (JIA) were studied. MRP8/MRP14 serum concentrations were determined by a sandwich enzyme linked immunosorbent assay (ELISA) as described. Improvement from baseline was described by a definition of improvement employing a core set of criteria as detailed previously by Giannini. Results: After ASCT, MRP8/MRP14 serum concentrations in JIA showed a positive correlation with the Child Health Assessment Questionnaire (CHAQ; r =0.80) and erythrocyte sedimentation rate ( r =0.45), but not with the total leucocyte count ( r =0.26). Mean MRP8/MRP14 serum concentrations dropped markedly in the first three months after ASCT (p=0.0039) and clinical parameters of disease activity such as CHAQ markedly improved (p=0.0039). During a transient relapse there was an increase in MRP8/MRP14. Conclusions: MRP8/MRP14 serum concentration can be used as a marker for disease activity in patients who receive an ASCT for refractory JIA. This indicates a role of macrophage activation in the pathogenesis of JIA. The occurrence of MAS in three patients in this study was not preceded by significant changes in MRP8/MRP14 concentration.